@inproceedings{wang_microrobotic_2008,
	title = {A {Microrobotic} {Adherent} {Cell} {Injection} {System} for {Investigating} {Intracellular} {Behavior} of {Quantum} {Dots}},
	doi = {10.1109/ROBOT.2008.4543241},
	abstract = {This paper presents a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision XY stage. The microrobotic system demonstrates an injection speed of 25 endothelial cells per minute with a survival rate of 96\% and a success rate of 82\% (n=1012). The system has a high degree of performance consistency. It is immune to operator proficiency variations and from human fatigue, requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic adherent cell injection system makes the injection of thousands of adherent cells practical and will enable our testing of intracellular behavior of semiconductive quantum dots (QDs).},
	booktitle = {2008 {IEEE} {International} {Conference} on {Robotics} and {Automation}},
	author = {Wang, W.H. and Sun, Y. and Zhang, M. and Anderson, R. and Langille, L. and Chan, W.},
	month = may,
	year = {2008},
	note = {ISSN: 1050-4729},
	keywords = {Adherent cell, Computer vision, Control systems, Embryo, endothelial cells, Humans, Immune system, microrobotic injection, Microscopy, molecule screening, Morphology, Motion control, quantum dots, Quantum dots, Shape},
	pages = {407--412},
	file = {IEEE Xplore Full Text PDF:files/2166/Wang et al. - 2008 - A Microrobotic Adherent Cell Injection System for .pdf:application/pdf;IEEE Xplore Abstract Record:files/2170/4543241.html:text/html},
}

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Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision XY stage. The microrobotic system demonstrates an injection speed of 25 endothelial cells per minute with a survival rate of 96% and a success rate of 82% (n=1012). The system has a high degree of performance consistency. It is immune to operator proficiency variations and from human fatigue, requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic adherent cell injection system makes the injection of thousands of adherent cells practical and will enable our testing of intracellular behavior of semiconductive quantum dots (QDs).","booktitle":"2008 IEEE International Conference on Robotics and Automation","author":[{"propositions":[],"lastnames":["Wang"],"firstnames":["W.H."],"suffixes":[]},{"propositions":[],"lastnames":["Sun"],"firstnames":["Y."],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Anderson"],"firstnames":["R."],"suffixes":[]},{"propositions":[],"lastnames":["Langille"],"firstnames":["L."],"suffixes":[]},{"propositions":[],"lastnames":["Chan"],"firstnames":["W."],"suffixes":[]}],"month":"May","year":"2008","note":"ISSN: 1050-4729","keywords":"Adherent cell, Computer vision, Control systems, Embryo, endothelial cells, Humans, Immune system, microrobotic injection, Microscopy, molecule screening, Morphology, Motion control, quantum dots, Quantum dots, Shape","pages":"407–412","file":"IEEE Xplore Full Text PDF:files/2166/Wang et al. - 2008 - A Microrobotic Adherent Cell Injection System for .pdf:application/pdf;IEEE Xplore Abstract Record:files/2170/4543241.html:text/html","bibtex":"@inproceedings{wang_microrobotic_2008,\n\ttitle = {A {Microrobotic} {Adherent} {Cell} {Injection} {System} for {Investigating} {Intracellular} {Behavior} of {Quantum} {Dots}},\n\tdoi = {10.1109/ROBOT.2008.4543241},\n\tabstract = {This paper presents a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision XY stage. The microrobotic system demonstrates an injection speed of 25 endothelial cells per minute with a survival rate of 96\\% and a success rate of 82\\% (n=1012). The system has a high degree of performance consistency. It is immune to operator proficiency variations and from human fatigue, requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic adherent cell injection system makes the injection of thousands of adherent cells practical and will enable our testing of intracellular behavior of semiconductive quantum dots (QDs).},\n\tbooktitle = {2008 {IEEE} {International} {Conference} on {Robotics} and {Automation}},\n\tauthor = {Wang, W.H. and Sun, Y. and Zhang, M. and Anderson, R. and Langille, L. and Chan, W.},\n\tmonth = may,\n\tyear = {2008},\n\tnote = {ISSN: 1050-4729},\n\tkeywords = {Adherent cell, Computer vision, Control systems, Embryo, endothelial cells, Humans, Immune system, microrobotic injection, Microscopy, molecule screening, Morphology, Motion control, quantum dots, Quantum dots, Shape},\n\tpages = {407--412},\n\tfile = {IEEE Xplore Full Text PDF:files/2166/Wang et al. - 2008 - A Microrobotic Adherent Cell Injection System for .pdf:application/pdf;IEEE Xplore Abstract Record:files/2170/4543241.html:text/html},\n}\n\n","author_short":["Wang, W.","Sun, Y.","Zhang, M.","Anderson, R.","Langille, L.","Chan, W."],"key":"wang_microrobotic_2008","id":"wang_microrobotic_2008","bibbaseid":"wang-sun-zhang-anderson-langille-chan-amicroroboticadherentcellinjectionsystemforinvestigatingintracellularbehaviorofquantumdots-2008","role":"author","urls":{},"keyword":["Adherent cell","Computer vision","Control systems","Embryo","endothelial cells","Humans","Immune system","microrobotic injection","Microscopy","molecule screening","Morphology","Motion control","quantum dots","Quantum dots","Shape"],"metadata":{"authorlinks":{}},"downloads":0,"html":""},"search_terms":["microrobotic","adherent","cell","injection","system","investigating","intracellular","behavior","quantum","dots","wang","sun","zhang","anderson","langille","chan"],"keywords":["adherent cell","computer vision","control systems","embryo","endothelial cells","humans","immune system","microrobotic injection","microscopy","molecule screening","morphology","motion control","quantum dots","quantum dots","shape"],"authorIDs":[],"dataSources":["SaMiCnbWbXAXFhxMg","zb3p4i4Fd9wXynbkF","pF7M3AvRzd8m3Nc5t","BqtRhZTYdfYNLhWzp","sBWiDMMhfDHZnN93b","zkBeHFgTXc62hytzj","s3xwaPmQ7EjjEcMSb","5npqWkz6DHBZLyn3J","yc5dXnBwovT8YvCrt","nLduyq7S9B96QB8B5","JiWrSgLBTKbDLqiYi"]}