A system for high-speed microinjection of adherent cells. Wang, W., Sun, Y., Zhang, M., Anderson, R., Langille, L., & Chan, W. Review of Scientific Instruments, 79(10):104302, October, 2008. Publisher: American Institute of Physics![A system for high-speed microinjection of adherent cells [link]](https://bibbase.org/img/filetypes/link.svg "link")
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Paper doi abstract bibtex This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision 𝑋𝑌 XY stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7% and a success rate of 82.4% (𝑛=1012) (n=1012) . The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.
@article{wang_system_2008,
title = {A system for high-speed microinjection of adherent cells},
volume = {79},
issn = {0034-6748},
url = {http://aip.scitation.org/doi/full/10.1063/1.3006000},
doi = {10.1063/1.3006000},
abstract = {This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision
𝑋𝑌
XY
stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7\% and a success rate of 82.4\%
(𝑛=1012)
(n=1012)
. The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.},
number = {10},
urldate = {2021-11-06},
journal = {Review of Scientific Instruments},
author = {Wang, Wenhui and Sun, Yu and Zhang, Ming and Anderson, Robin and Langille, Lowell and Chan, Warren},
month = oct,
year = {2008},
note = {Publisher: American Institute of Physics},
pages = {104302},
file = {Full Text PDF:files/2184/Wang et al. - 2008 - A system for high-speed microinjection of adherent.pdf:application/pdf},
url_Paper = {https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/1.3006000.pdf}
}