@article{wang_system_2008,
	title = {A system for high-speed microinjection of adherent cells},
	volume = {79},
	issn = {0034-6748},
	url = {http://aip.scitation.org/doi/full/10.1063/1.3006000},
	doi = {10.1063/1.3006000},
	abstract = {This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision 
𝑋𝑌
XY
 stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7\% and a success rate of 82.4\% 
(𝑛=1012)
(n=1012)
. The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.},
	number = {10},
	urldate = {2021-11-06},
	journal = {Review of Scientific Instruments},
	author = {Wang, Wenhui and Sun, Yu and Zhang, Ming and Anderson, Robin and Langille, Lowell and Chan, Warren},
	month = oct,
	year = {2008},
	note = {Publisher: American Institute of Physics},
	pages = {104302},
	file = {Full Text PDF:files/2184/Wang et al. - 2008 - A system for high-speed microinjection of adherent.pdf:application/pdf},
	url_Paper = {https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/1.3006000.pdf}
}

{"_id":"jdFvkCBhNh46xwyCP","bibbaseid":"wang-sun-zhang-anderson-langille-chan-asystemforhighspeedmicroinjectionofadherentcells-2008","author_short":["Wang, W.","Sun, Y.","Zhang, M.","Anderson, R.","Langille, L.","Chan, W."],"bibdata":{"bibtype":"article","type":"article","title":"A system for high-speed microinjection of adherent cells","volume":"79","issn":"0034-6748","url":"http://aip.scitation.org/doi/full/10.1063/1.3006000","doi":"10.1063/1.3006000","abstract":"This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision 𝑋𝑌 XY stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7% and a success rate of 82.4% (𝑛=1012) (n=1012) . The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.","number":"10","urldate":"2021-11-06","journal":"Review of Scientific Instruments","author":[{"propositions":[],"lastnames":["Wang"],"firstnames":["Wenhui"],"suffixes":[]},{"propositions":[],"lastnames":["Sun"],"firstnames":["Yu"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Ming"],"suffixes":[]},{"propositions":[],"lastnames":["Anderson"],"firstnames":["Robin"],"suffixes":[]},{"propositions":[],"lastnames":["Langille"],"firstnames":["Lowell"],"suffixes":[]},{"propositions":[],"lastnames":["Chan"],"firstnames":["Warren"],"suffixes":[]}],"month":"October","year":"2008","note":"Publisher: American Institute of Physics","pages":"104302","file":"Full Text PDF:files/2184/Wang et al. - 2008 - A system for high-speed microinjection of adherent.pdf:application/pdf","url_paper":"https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/1.3006000.pdf","bibtex":"@article{wang_system_2008,\n\ttitle = {A system for high-speed microinjection of adherent cells},\n\tvolume = {79},\n\tissn = {0034-6748},\n\turl = {http://aip.scitation.org/doi/full/10.1063/1.3006000},\n\tdoi = {10.1063/1.3006000},\n\tabstract = {This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision microscopy and motion control, the system coordinately controls a three-degrees-of-freedom microrobot and a precision \n𝑋𝑌\nXY\n stage, demonstrating an injection speed of 25 endothelial cells per minute with a survival rate of 95.7\\% and a success rate of 82.4\\% \n(𝑛=1012)\n(n=1012)\n. The system has a high degree of performance consistency. It is operator skill independent and immune from human fatigue, only requiring a human operator to select injection destinations through computer mouse clicking as the only operator intervention. The microrobotic system makes the injection of a large number of adherent cells practical for testing cellular responses to foreign molecules.},\n\tnumber = {10},\n\turldate = {2021-11-06},\n\tjournal = {Review of Scientific Instruments},\n\tauthor = {Wang, Wenhui and Sun, Yu and Zhang, Ming and Anderson, Robin and Langille, Lowell and Chan, Warren},\n\tmonth = oct,\n\tyear = {2008},\n\tnote = {Publisher: American Institute of Physics},\n\tpages = {104302},\n\tfile = {Full Text PDF:files/2184/Wang et al. - 2008 - A system for high-speed microinjection of adherent.pdf:application/pdf},\n\turl_Paper = {https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/1.3006000.pdf}\n}\n\n","author_short":["Wang, W.","Sun, Y.","Zhang, M.","Anderson, R.","Langille, L.","Chan, W."],"key":"wang_system_2008","id":"wang_system_2008","bibbaseid":"wang-sun-zhang-anderson-langille-chan-asystemforhighspeedmicroinjectionofadherentcells-2008","role":"author","urls":{"Paper":"http://aip.scitation.org/doi/full/10.1063/1.3006000"," paper":"https://inbs.med.utoronto.ca/wp-content/uploads/2020/08/1.3006000.pdf"},"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://inbslab.com//wp-content/uploads/2024/03/Chan-2024_03-14.txt","dataSources":["zb3p4i4Fd9wXynbkF","pF7M3AvRzd8m3Nc5t","BqtRhZTYdfYNLhWzp","sBWiDMMhfDHZnN93b","zkBeHFgTXc62hytzj","s3xwaPmQ7EjjEcMSb","5npqWkz6DHBZLyn3J","yc5dXnBwovT8YvCrt","nLduyq7S9B96QB8B5","JiWrSgLBTKbDLqiYi"],"keywords":[],"search_terms":["system","high","speed","microinjection","adherent","cells","wang","sun","zhang","anderson","langille","chan"],"title":"A system for high-speed microinjection of adherent cells","year":2008}