Sensing wetness: a new role for the bacterial flagellum. Wang, Q., Suzuki, A., Mariconda, S., Porwollik, S., & Harshey, R. M The EMBO Journal, 24(11):2034--2042, 2005. ![Sensing wetness: a new role for the bacterial flagellum [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex We have uncovered a new role for the bacterial flagellum in sensing external wetness. An investigation into why mutants in the chemotaxis signaling pathway of Salmonella typhimurium exhibit fewer and shorter flagella than wild-type when propagated on a surface, first showed that the mutants downregulate only a small set of genes on swarm media—class 3 or ‘late’ motility genes, and genes associated with the pathogenicity island SPI-1 TTSS (type three secretion system). Based on observations that swarm colonies of the mutants appear less hydrated, we tested a model in which the flagellum itself is a sensor: suboptimal external hydration interferes with secretion of flagellin subunits, inhibiting filament growth and blocking normal export of the class 3 transcription inhibitor FlgM. We provide strong experimental support for the model. In addition, the data show that the flagellar and SPI-1 TTSS are coupled via regulatory proteins. These studies implicate the flagellum, a bacterial organ for motility, in sensing the external environment to modulate not only its own biogenesis but other physiological functions as well.
@article{wang_sensing_2005,
title = {Sensing wetness: a new role for the bacterial flagellum},
volume = {24},
copyright = {Copyright © 2005 European Molecular Biology Organization},
issn = {1460-2075},
shorttitle = {Sensing wetness},
url = {http://onlinelibrary.wiley.com/doi/10.1038/sj.emboj.7600668/abstract},
doi = {10.1038/sj.emboj.7600668},
abstract = {We have uncovered a new role for the bacterial flagellum in sensing external wetness. An investigation into why mutants in the chemotaxis signaling pathway of Salmonella typhimurium exhibit fewer and shorter flagella than wild-type when propagated on a surface, first showed that the mutants downregulate only a small set of genes on swarm media—class 3 or ‘late’ motility genes, and genes associated with the pathogenicity island SPI-1 TTSS (type three secretion system). Based on observations that swarm colonies of the mutants appear less hydrated, we tested a model in which the flagellum itself is a sensor: suboptimal external hydration interferes with secretion of flagellin subunits, inhibiting filament growth and blocking normal export of the class 3 transcription inhibitor FlgM. We provide strong experimental support for the model. In addition, the data show that the flagellar and SPI-1 TTSS are coupled via regulatory proteins. These studies implicate the flagellum, a bacterial organ for motility, in sensing the external environment to modulate not only its own biogenesis but other physiological functions as well.},
language = {en},
number = {11},
urldate = {2014-12-30TZ},
journal = {The EMBO Journal},
author = {Wang, Qingfeng and Suzuki, Asaka and Mariconda, Susana and Porwollik, Steffen and Harshey, Rasika M},
year = {2005},
keywords = {Salmonella pathogenicity island, chemotaxis signaling pathway, flagella biogenesis, flagellum as sensor, swarming motility},
pages = {2034--2042}
}
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