@article{warren_differentiation_2006,
	title = {Differentiation of {Mycobacterium} tuberculosis complex by {PCR} amplification of genomic regions of difference},
	volume = {10},
	issn = {1027-3719},
	abstract = {Differentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.},
	language = {eng},
	number = {7},
	journal = {The International Journal of Tuberculosis and Lung Disease: The Official Journal of the International Union Against Tuberculosis and Lung Disease},
	author = {Warren, R. M. and Gey van Pittius, N. C. and Barnard, M. and Hesseling, A. and Engelke, E. and de Kock, M. and Gutierrez, M. C. and Chege, G. K. and Victor, T. C. and Hoal, E. G. and van Helden, P. D.},
	month = jul,
	year = {2006},
	pmid = {16850559},
	note = {00118 },
	keywords = {Electrophoresis, Polyacrylamide Gel, Genes, Bacterial, Mycobacterium tuberculosis, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Species Specificity},
	pages = {818--822},
}

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A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.","language":"eng","number":"7","journal":"The International Journal of Tuberculosis and Lung Disease: The Official Journal of the International Union Against Tuberculosis and Lung Disease","author":[{"propositions":[],"lastnames":["Warren"],"firstnames":["R.","M."],"suffixes":[]},{"propositions":["Gey","van"],"lastnames":["Pittius"],"firstnames":["N.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Barnard"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Hesseling"],"firstnames":["A."],"suffixes":[]},{"propositions":[],"lastnames":["Engelke"],"firstnames":["E."],"suffixes":[]},{"propositions":["de"],"lastnames":["Kock"],"firstnames":["M."],"suffixes":[]},{"propositions":[],"lastnames":["Gutierrez"],"firstnames":["M.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Chege"],"firstnames":["G.","K."],"suffixes":[]},{"propositions":[],"lastnames":["Victor"],"firstnames":["T.","C."],"suffixes":[]},{"propositions":[],"lastnames":["Hoal"],"firstnames":["E.","G."],"suffixes":[]},{"propositions":["van"],"lastnames":["Helden"],"firstnames":["P.","D."],"suffixes":[]}],"month":"July","year":"2006","pmid":"16850559","note":"00118 ","keywords":"Electrophoresis, Polyacrylamide Gel, Genes, Bacterial, Mycobacterium tuberculosis, Polymerase Chain Reaction, RNA, Ribosomal, 16S, Species Specificity","pages":"818–822","bibtex":"@article{warren_differentiation_2006,\n\ttitle = {Differentiation of {Mycobacterium} tuberculosis complex by {PCR} amplification of genomic regions of difference},\n\tvolume = {10},\n\tissn = {1027-3719},\n\tabstract = {Differentiation of members of the Mycobacterium tuberculosis complex by conventional mycobacteriological methods is time consuming, making surveillance of species-specific disease difficult. A two-step, multiplex polymerase chain reaction (PCR) method based on genomic regions of difference (RD1, RD1(mic), RD2(seal), RD4, RD9 and RD12) was developed for the differentiation of M. canettii, M. tuberculosis, M. africanum, M. microti, M. pinnipedii, M. caprae, M. bovis and M. bovis BCG. The size of the respective multiplex PCR amplification products corresponded to the presence of the different M. tuberculosis complex members. This method allows for rapid differentiation, making it suitable for routine laboratories and surveillance purposes.},\n\tlanguage = {eng},\n\tnumber = {7},\n\tjournal = {The International Journal of Tuberculosis and Lung Disease: The Official Journal of the International Union Against Tuberculosis and Lung Disease},\n\tauthor = {Warren, R. M. and Gey van Pittius, N. C. and Barnard, M. and Hesseling, A. and Engelke, E. and de Kock, M. and Gutierrez, M. C. and Chege, G. K. and Victor, T. C. and Hoal, E. G. and van Helden, P. 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D."],"key":"warren_differentiation_2006","id":"warren_differentiation_2006","bibbaseid":"warren-geyvanpittius-barnard-hesseling-engelke-dekock-gutierrez-chege-etal-differentiationofmycobacteriumtuberculosiscomplexbypcramplificationofgenomicregionsofdifference-2006","role":"author","urls":{},"keyword":["Electrophoresis","Polyacrylamide Gel","Genes","Bacterial","Mycobacterium tuberculosis","Polymerase Chain Reaction","RNA","Ribosomal","16S","Species Specificity"],"metadata":{"authorlinks":{}},"downloads":0,"html":""},"bibtype":"article","biburl":"http://bibbase.org/zotero/TBHostGen","dataSources":["csPxbBZ9RG8CqYnrf"],"keywords":["electrophoresis","polyacrylamide gel","genes","bacterial","mycobacterium tuberculosis","polymerase chain reaction","rna","ribosomal","16s","species specificity"],"search_terms":["differentiation","mycobacterium","tuberculosis","complex","pcr","amplification","genomic","regions","difference","warren","gey van pittius","barnard","hesseling","engelke","de kock","gutierrez","chege","victor","hoal","van helden"],"title":"Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference","year":2006}