Evidence for the binding mode of porphyrins to G-quadruplex DNA. Wei, C., Jia, G., Zhou, J., Han, G., & Li, C. Physical chemistry chemical physics : PCCP, 11(20):4025–32, May, 2009. ISBN: 8641184694![Evidence for the binding mode of porphyrins to G-quadruplex DNA. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Interactions of porphyrin derivatives 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with human telomeric AG(3)(T(2)AG(3))(3) G-quadruplex DNAs in 150 mM K(+)-containing buffer in the presence or absence of 40% molecular crowding agent poly(ethylene glycol) (PEG 200) were studied by absorption titration fitting and time-resolved fluorescence spectroscopy. The results show that two TMPyP4 (or TPrPyP4) molecules bind to antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3) G-quadruplex by end-stacking and outside groove binding modes in the absence of PEG. Interestingly, in the presence of PEG one porphyrin molecule is stacked between two parallel AG(3)(T(2)AG(3))(3) G- quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the G-quadruplex. The interactions of TMPyP4 with different structures of AG(3)(T(2)AG(3))(3) G-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) M(-1) for an antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3), 8.67 x 10(5) and 2.26 x 10(8) M(-1) for parallel-stranded AG(3)(T(2)AG(3))(3) G-quadruplex. Conversely, the two binding sites are cooperative for TPrPyP4, the apparent association constants are 5.58 x 10(6) and 1.24 x 10(7) M(-1) for parallel-stranded and antiparallel/parallel hybrid structures of AG(3)(T(2)AG(3))(3) G-quadruplex, respectively.
@article{Wei2009,
title = {Evidence for the binding mode of porphyrins to {G}-quadruplex {DNA}.},
volume = {11},
issn = {1463-9076},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19440632},
doi = {10.1039/b901027k},
abstract = {Interactions of porphyrin derivatives 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with human telomeric AG(3)(T(2)AG(3))(3) G-quadruplex DNAs in 150 mM K(+)-containing buffer in the presence or absence of 40\% molecular crowding agent poly(ethylene glycol) (PEG 200) were studied by absorption titration fitting and time-resolved fluorescence spectroscopy. The results show that two TMPyP4 (or TPrPyP4) molecules bind to antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3) G-quadruplex by end-stacking and outside groove binding modes in the absence of PEG. Interestingly, in the presence of PEG one porphyrin molecule is stacked between two parallel AG(3)(T(2)AG(3))(3) G- quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the G-quadruplex. The interactions of TMPyP4 with different structures of AG(3)(T(2)AG(3))(3) G-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) M(-1) for an antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3), 8.67 x 10(5) and 2.26 x 10(8) M(-1) for parallel-stranded AG(3)(T(2)AG(3))(3) G-quadruplex. Conversely, the two binding sites are cooperative for TPrPyP4, the apparent association constants are 5.58 x 10(6) and 1.24 x 10(7) M(-1) for parallel-stranded and antiparallel/parallel hybrid structures of AG(3)(T(2)AG(3))(3) G-quadruplex, respectively.},
number = {20},
journal = {Physical chemistry chemical physics : PCCP},
author = {Wei, Chunying and Jia, Guoqing and Zhou, Jun and Han, Gaoyi and Li, Can},
month = may,
year = {2009},
pmid = {19440632},
note = {ISBN: 8641184694},
keywords = {\#nosource, Absorption, Buffers, Circular Dichroism, DNA, DNA: chemistry, Fluorescence, G-Quadruplexes, Humans, Melanins, Polyethylene Glycols, Polyethylene Glycols: chemistry, Porphyrins, Porphyrins: chemistry, Spectrometry},
pages = {4025--32},
}
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The results show that two TMPyP4 (or TPrPyP4) molecules bind to antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3) G-quadruplex by end-stacking and outside groove binding modes in the absence of PEG. Interestingly, in the presence of PEG one porphyrin molecule is stacked between two parallel AG(3)(T(2)AG(3))(3) G- quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the G-quadruplex. The interactions of TMPyP4 with different structures of AG(3)(T(2)AG(3))(3) G-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) M(-1) for an antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3), 8.67 x 10(5) and 2.26 x 10(8) M(-1) for parallel-stranded AG(3)(T(2)AG(3))(3) G-quadruplex. Conversely, the two binding sites are cooperative for TPrPyP4, the apparent association constants are 5.58 x 10(6) and 1.24 x 10(7) M(-1) for parallel-stranded and antiparallel/parallel hybrid structures of AG(3)(T(2)AG(3))(3) G-quadruplex, respectively.","number":"20","journal":"Physical chemistry chemical physics : PCCP","author":[{"propositions":[],"lastnames":["Wei"],"firstnames":["Chunying"],"suffixes":[]},{"propositions":[],"lastnames":["Jia"],"firstnames":["Guoqing"],"suffixes":[]},{"propositions":[],"lastnames":["Zhou"],"firstnames":["Jun"],"suffixes":[]},{"propositions":[],"lastnames":["Han"],"firstnames":["Gaoyi"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Can"],"suffixes":[]}],"month":"May","year":"2009","pmid":"19440632","note":"ISBN: 8641184694","keywords":"#nosource, Absorption, Buffers, Circular Dichroism, DNA, DNA: chemistry, Fluorescence, G-Quadruplexes, Humans, Melanins, Polyethylene Glycols, Polyethylene Glycols: chemistry, Porphyrins, Porphyrins: chemistry, Spectrometry","pages":"4025–32","bibtex":"@article{Wei2009,\n\ttitle = {Evidence for the binding mode of porphyrins to {G}-quadruplex {DNA}.},\n\tvolume = {11},\n\tissn = {1463-9076},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/19440632},\n\tdoi = {10.1039/b901027k},\n\tabstract = {Interactions of porphyrin derivatives 5,10,15,20-tetrakis(N-methylpyridinium-4-yl)-21H,23H-porphyrin (TMPyP4) and 5,10,15,20-tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with human telomeric AG(3)(T(2)AG(3))(3) G-quadruplex DNAs in 150 mM K(+)-containing buffer in the presence or absence of 40\\% molecular crowding agent poly(ethylene glycol) (PEG 200) were studied by absorption titration fitting and time-resolved fluorescence spectroscopy. The results show that two TMPyP4 (or TPrPyP4) molecules bind to antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3) G-quadruplex by end-stacking and outside groove binding modes in the absence of PEG. Interestingly, in the presence of PEG one porphyrin molecule is stacked between two parallel AG(3)(T(2)AG(3))(3) G- quadruplexes to form a sandwich structure, another porphyrin molecule is bound to the groove of the G-quadruplex. The interactions of TMPyP4 with different structures of AG(3)(T(2)AG(3))(3) G-quadruplex are non cooperative, the binding constants of two independent binding sites are 1.07 x 10(6) and 4.42 x 10(8) M(-1) for an antiparallel/parallel hybrid structure of AG(3)(T(2)AG(3))(3), 8.67 x 10(5) and 2.26 x 10(8) M(-1) for parallel-stranded AG(3)(T(2)AG(3))(3) G-quadruplex. 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