@article{wu_comparative_nodate,
	title = {Comparative {Analysis} and {Refinement} of {Human} {PSC}-{Derived} {Kidney} {Organoid} {Differentiation} with {Single}-{Cell} {Transcriptomics}},
	issn = {1934-5909},
	url = {https://doi.org/10.1016/j.stem.2018.10.010},
	doi = {10.1016/j.stem.2018.10.010},
	abstract = {Kidney organoids derived from human pluripotent stem cells have great utility for investigating organogenesis and disease mechanisms and, potentially, as a replacement tissue source, but how closely organoids derived from current protocols replicate adult human kidney is undefined. We compared two directed differentiation protocols by single-cell transcriptomics of 83,130 cells from 65 organoids with single-cell transcriptomes of fetal and adult kidney cells. Both protocols generate a diverse range of kidney cells with differing ratios, but organoid-derived cell types are immature, and 10\%?20\% of cells are non-renal. Reconstructing lineage relationships by pseudotemporal ordering identified ligands, receptors, and transcription factor networks associated with fate decisions. Brain-derived neurotrophic factor (BDNF) and its cognate receptor NTRK2 were expressed in the neuronal lineage during organoid differentiation. Inhibiting this pathway improved organoid formation by reducing neurons by 90\% without affecting kidney differentiation, highlighting the power of single-cell technologies to characterize and improve organoid differentiation.},
	urldate = {2018-11-15},
	journal = {Cell Stem Cell},
	author = {Wu, Haojia and Uchimura, Kohei and Donnelly, Erinn L. and Kirita, Yuhei and Morris, Samantha A. and Humphreys, Benjamin D.},
}

{"_id":"2Y6sTTeyXfvkCEgh8","bibbaseid":"wu-uchimura-donnelly-kirita-morris-humphreys-comparativeanalysisandrefinementofhumanpscderivedkidneyorganoiddifferentiationwithsinglecelltranscriptomics","downloads":0,"creationDate":"2018-11-30T20:21:59.816Z","title":"Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics","author_short":["Wu, H.","Uchimura, K.","Donnelly, E. L.","Kirita, Y.","Morris, S. A.","Humphreys, B. D."],"year":null,"bibtype":"article","biburl":"https://api.zotero.org/users/3649949/collections/KXIPVG5M/items?key=Ek345vndMXpZ41Qp4ndqP8RS&format=bibtex&limit=100","bibdata":{"bibtype":"article","type":"article","title":"Comparative Analysis and Refinement of Human PSC-Derived Kidney Organoid Differentiation with Single-Cell Transcriptomics","issn":"1934-5909","url":"https://doi.org/10.1016/j.stem.2018.10.010","doi":"10.1016/j.stem.2018.10.010","abstract":"Kidney organoids derived from human pluripotent stem cells have great utility for investigating organogenesis and disease mechanisms and, potentially, as a replacement tissue source, but how closely organoids derived from current protocols replicate adult human kidney is undefined. We compared two directed differentiation protocols by single-cell transcriptomics of 83,130 cells from 65 organoids with single-cell transcriptomes of fetal and adult kidney cells. Both protocols generate a diverse range of kidney cells with differing ratios, but organoid-derived cell types are immature, and 10%?20% of cells are non-renal. Reconstructing lineage relationships by pseudotemporal ordering identified ligands, receptors, and transcription factor networks associated with fate decisions. Brain-derived neurotrophic factor (BDNF) and its cognate receptor NTRK2 were expressed in the neuronal lineage during organoid differentiation. Inhibiting this pathway improved organoid formation by reducing neurons by 90% without affecting kidney differentiation, highlighting the power of single-cell technologies to characterize and improve organoid differentiation.","urldate":"2018-11-15","journal":"Cell Stem Cell","author":[{"propositions":[],"lastnames":["Wu"],"firstnames":["Haojia"],"suffixes":[]},{"propositions":[],"lastnames":["Uchimura"],"firstnames":["Kohei"],"suffixes":[]},{"propositions":[],"lastnames":["Donnelly"],"firstnames":["Erinn","L."],"suffixes":[]},{"propositions":[],"lastnames":["Kirita"],"firstnames":["Yuhei"],"suffixes":[]},{"propositions":[],"lastnames":["Morris"],"firstnames":["Samantha","A."],"suffixes":[]},{"propositions":[],"lastnames":["Humphreys"],"firstnames":["Benjamin","D."],"suffixes":[]}],"bibtex":"@article{wu_comparative_nodate,\n\ttitle = {Comparative {Analysis} and {Refinement} of {Human} {PSC}-{Derived} {Kidney} {Organoid} {Differentiation} with {Single}-{Cell} {Transcriptomics}},\n\tissn = {1934-5909},\n\turl = {https://doi.org/10.1016/j.stem.2018.10.010},\n\tdoi = {10.1016/j.stem.2018.10.010},\n\tabstract = {Kidney organoids derived from human pluripotent stem cells have great utility for investigating organogenesis and disease mechanisms and, potentially, as a replacement tissue source, but how closely organoids derived from current protocols replicate adult human kidney is undefined. We compared two directed differentiation protocols by single-cell transcriptomics of 83,130 cells from 65 organoids with single-cell transcriptomes of fetal and adult kidney cells. Both protocols generate a diverse range of kidney cells with differing ratios, but organoid-derived cell types are immature, and 10\\%?20\\% of cells are non-renal. Reconstructing lineage relationships by pseudotemporal ordering identified ligands, receptors, and transcription factor networks associated with fate decisions. Brain-derived neurotrophic factor (BDNF) and its cognate receptor NTRK2 were expressed in the neuronal lineage during organoid differentiation. Inhibiting this pathway improved organoid formation by reducing neurons by 90\\% without affecting kidney differentiation, highlighting the power of single-cell technologies to characterize and improve organoid differentiation.},\n\turldate = {2018-11-15},\n\tjournal = {Cell Stem Cell},\n\tauthor = {Wu, Haojia and Uchimura, Kohei and Donnelly, Erinn L. and Kirita, Yuhei and Morris, Samantha A. and Humphreys, Benjamin D.},\n}\n\n","author_short":["Wu, H.","Uchimura, K.","Donnelly, E. L.","Kirita, Y.","Morris, S. A.","Humphreys, B. D."],"key":"wu_comparative_nodate","id":"wu_comparative_nodate","bibbaseid":"wu-uchimura-donnelly-kirita-morris-humphreys-comparativeanalysisandrefinementofhumanpscderivedkidneyorganoiddifferentiationwithsinglecelltranscriptomics","role":"author","urls":{"Paper":"https://doi.org/10.1016/j.stem.2018.10.010"},"metadata":{"authorlinks":{}}},"search_terms":["comparative","analysis","refinement","human","psc","derived","kidney","organoid","differentiation","single","cell","transcriptomics","wu","uchimura","donnelly","kirita","morris","humphreys"],"keywords":[],"authorIDs":[],"dataSources":["7HaEtSSZCgGAu2Lkg"]}