Bifacial peptide nucleic acid as an allosteric switch for aptamer and ribozyme function. Xia, X., Piao, X., & Bong, D. Journal of the American Chemical Society, 136(20):7265–8, May, 2014. ![Bifacial peptide nucleic acid as an allosteric switch for aptamer and ribozyme function. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex We demonstrate herein that bifacial peptide nucleic acid (bPNA) hybrid triplexes functionally substitute for duplex DNA or RNA. Structure-function loss in three non-coding nucleic acids was inflicted by replacement of a duplex stem with unstructured oligo-T/U strands, which are bPNA binding sites. Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hybrid stems. Bifacial PNA binding was thus used to allosterically switch-on protein and small-molecule binding in DNA and RNA aptamers, as well as catalytic bond scission in a ribozyme. Duplex stems that support the catalytic site of a minimal type I hammerhead ribozyme were replaced with oligo-U loops, severely crippling or ablating the native RNA splicing function. Refolding of the U-loops into bPNA triplex stems completely restored splicing function in the hybrid system. These studies indicate that bPNA may have general utility as an allosteric trigger for a wide range of functions in non-coding nucleic acids.
@article{Xia2014,
title = {Bifacial peptide nucleic acid as an allosteric switch for aptamer and ribozyme function.},
volume = {136},
issn = {1520-5126},
url = {http://www.ncbi.nlm.nih.gov/pubmed/24796374},
doi = {10.1021/ja5032584},
abstract = {We demonstrate herein that bifacial peptide nucleic acid (bPNA) hybrid triplexes functionally substitute for duplex DNA or RNA. Structure-function loss in three non-coding nucleic acids was inflicted by replacement of a duplex stem with unstructured oligo-T/U strands, which are bPNA binding sites. Functional rescue was observed on refolding of the oligo-T/U strands into bPNA triplex hybrid stems. Bifacial PNA binding was thus used to allosterically switch-on protein and small-molecule binding in DNA and RNA aptamers, as well as catalytic bond scission in a ribozyme. Duplex stems that support the catalytic site of a minimal type I hammerhead ribozyme were replaced with oligo-U loops, severely crippling or ablating the native RNA splicing function. Refolding of the U-loops into bPNA triplex stems completely restored splicing function in the hybrid system. These studies indicate that bPNA may have general utility as an allosteric trigger for a wide range of functions in non-coding nucleic acids.},
number = {20},
journal = {Journal of the American Chemical Society},
author = {Xia, Xin and Piao, Xijun and Bong, Dennis},
month = may,
year = {2014},
pmid = {24796374},
keywords = {\#nosource},
pages = {7265--8},
}
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