{"_id":"NsKk56pjXKLbGuEhr","bibbaseid":"xiao-zhang-wu-wu-xia-zheng-hao-zhou-etal-formationofdnarnahybridgquadruplexesoftwogquartetlayersintranscriptionexpansionoftheprevalenceanddiversityofgquadruplexesingenomes-2014","author_short":["Xiao, S.","Zhang, J.","Wu, J.","Wu, R.","Xia, Y.","Zheng, K.","Hao, Y.","Zhou, X.","Tan, Z."],"bibdata":{"bibtype":"article","type":"article","title":"Formation of DNA:RNA hybrid g-quadruplexes of two g-quartet layers in transcription: Expansion of the prevalence and diversity of g-quadruplexes in genomes","volume":"53","issn":"14337851","url":"http://www.ncbi.nlm.nih.gov/pubmed/25267250 http://doi.wiley.com/10.1002/anie.201407045","doi":"10.1002/anie.201407045","abstract":"G-quadruplexes are implicated in important cellular processes. Previous studies mostly focused on intramolecular G-quadruplexes of three or more G-quartets. Those composed of two G-quartets were only shown to form in single-stranded oligonucleotides. On the basis of electrophoresis, DMS footprinting, fluorescence labeling, and photo-cross-linking, we detected the formation of DNA:RNA hybrid G-quadruplexes (HQs) of two G-quartets during the transcription of DNA duplexes. These HQs have a lifetime on the minute scale and are stabilized by a stabilizing ligand. They are far shorter-lived than the HQs of three G-quartets, which last for hours. The occurrence of putative formation motifs of such HQs shows a transcription-dependent strand-biased selection, thus supporting their formation and function in genomes. They are present in almost all human genes in large amounts. We speculate that the two-G-quartet HQs may be a distinct type of G-quadruplexes that may play a role in timely responsive processes and for purposes of fine-tuning.","number":"48","journal":"Angewandte Chemie International Edition","author":[{"propositions":[],"lastnames":["Xiao"],"firstnames":["Shan"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Jia-Yu"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["Jun"],"suffixes":[]},{"propositions":[],"lastnames":["Wu"],"firstnames":["Ren-Yi"],"suffixes":[]},{"propositions":[],"lastnames":["Xia"],"firstnames":["Ye"],"suffixes":[]},{"propositions":[],"lastnames":["Zheng"],"firstnames":["Ke-Wei"],"suffixes":[]},{"propositions":[],"lastnames":["Hao"],"firstnames":["Yu-Hua"],"suffixes":[]},{"propositions":[],"lastnames":["Zhou"],"firstnames":["Xiang"],"suffixes":[]},{"propositions":[],"lastnames":["Tan"],"firstnames":["Zheng"],"suffixes":[]}],"month":"November","year":"2014","pmid":"25267250","keywords":"#nosource","pages":"13110–13114","bibtex":"@article{Xiao2014,\n\ttitle = {Formation of {DNA}:{RNA} hybrid g-quadruplexes of two g-quartet layers in transcription: {Expansion} of the prevalence and diversity of g-quadruplexes in genomes},\n\tvolume = {53},\n\tissn = {14337851},\n\turl = {http://www.ncbi.nlm.nih.gov/pubmed/25267250 http://doi.wiley.com/10.1002/anie.201407045},\n\tdoi = {10.1002/anie.201407045},\n\tabstract = {G-quadruplexes are implicated in important cellular processes. Previous studies mostly focused on intramolecular G-quadruplexes of three or more G-quartets. Those composed of two G-quartets were only shown to form in single-stranded oligonucleotides. On the basis of electrophoresis, DMS footprinting, fluorescence labeling, and photo-cross-linking, we detected the formation of DNA:RNA hybrid G-quadruplexes (HQs) of two G-quartets during the transcription of DNA duplexes. These HQs have a lifetime on the minute scale and are stabilized by a stabilizing ligand. They are far shorter-lived than the HQs of three G-quartets, which last for hours. The occurrence of putative formation motifs of such HQs shows a transcription-dependent strand-biased selection, thus supporting their formation and function in genomes. They are present in almost all human genes in large amounts. We speculate that the two-G-quartet HQs may be a distinct type of G-quadruplexes that may play a role in timely responsive processes and for purposes of fine-tuning.},\n\tnumber = {48},\n\tjournal = {Angewandte Chemie International Edition},\n\tauthor = {Xiao, Shan and Zhang, Jia-Yu and Wu, Jun and Wu, Ren-Yi and Xia, Ye and Zheng, Ke-Wei and Hao, Yu-Hua and Zhou, Xiang and Tan, Zheng},\n\tmonth = nov,\n\tyear = {2014},\n\tpmid = {25267250},\n\tkeywords = {\\#nosource},\n\tpages = {13110--13114},\n}\n\n","author_short":["Xiao, S.","Zhang, J.","Wu, J.","Wu, R.","Xia, Y.","Zheng, K.","Hao, Y.","Zhou, X.","Tan, Z."],"key":"Xiao2014","id":"Xiao2014","bibbaseid":"xiao-zhang-wu-wu-xia-zheng-hao-zhou-etal-formationofdnarnahybridgquadruplexesoftwogquartetlayersintranscriptionexpansionoftheprevalenceanddiversityofgquadruplexesingenomes-2014","role":"author","urls":{"Paper":"http://www.ncbi.nlm.nih.gov/pubmed/25267250 http://doi.wiley.com/10.1002/anie.201407045"},"keyword":["#nosource"],"metadata":{"authorlinks":{}},"html":""},"bibtype":"article","biburl":"https://bibbase.org/zotero/eric.larG4","dataSources":["4i5C7S78DvJNsaHyg","5L2zM5wNE5CBYNuea"],"keywords":["#nosource"],"search_terms":["formation","dna","rna","hybrid","quadruplexes","two","quartet","layers","transcription","expansion","prevalence","diversity","quadruplexes","genomes","xiao","zhang","wu","wu","xia","zheng","hao","zhou","tan"],"title":"Formation of DNA:RNA hybrid g-quadruplexes of two g-quartet layers in transcription: Expansion of the prevalence and diversity of g-quadruplexes in genomes","year":2014}