Formation of DNA:RNA hybrid g-quadruplexes of two g-quartet layers in transcription: Expansion of the prevalence and diversity of g-quadruplexes in genomes

Formation of DNA:RNA hybrid g-quadruplexes of two g-quartet layers in transcription: Expansion of the prevalence and diversity of g-quadruplexes in genomes. Xiao, S., Zhang, J., Wu, J., Wu, R., Xia, Y., Zheng, K., Hao, Y., Zhou, X., & Tan, Z. Angewandte Chemie International Edition, 53(48):13110–13114, November, 2014.

Paper doi abstract bibtex

G-quadruplexes are implicated in important cellular processes. Previous studies mostly focused on intramolecular G-quadruplexes of three or more G-quartets. Those composed of two G-quartets were only shown to form in single-stranded oligonucleotides. On the basis of electrophoresis, DMS footprinting, fluorescence labeling, and photo-cross-linking, we detected the formation of DNA:RNA hybrid G-quadruplexes (HQs) of two G-quartets during the transcription of DNA duplexes. These HQs have a lifetime on the minute scale and are stabilized by a stabilizing ligand. They are far shorter-lived than the HQs of three G-quartets, which last for hours. The occurrence of putative formation motifs of such HQs shows a transcription-dependent strand-biased selection, thus supporting their formation and function in genomes. They are present in almost all human genes in large amounts. We speculate that the two-G-quartet HQs may be a distinct type of G-quadruplexes that may play a role in timely responsive processes and for purposes of fine-tuning.

@article{Xiao2014,
	title = {Formation of {DNA}:{RNA} hybrid g-quadruplexes of two g-quartet layers in transcription: {Expansion} of the prevalence and diversity of g-quadruplexes in genomes},
	volume = {53},
	issn = {14337851},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/25267250 http://doi.wiley.com/10.1002/anie.201407045},
	doi = {10.1002/anie.201407045},
	abstract = {G-quadruplexes are implicated in important cellular processes. Previous studies mostly focused on intramolecular G-quadruplexes of three or more G-quartets. Those composed of two G-quartets were only shown to form in single-stranded oligonucleotides. On the basis of electrophoresis, DMS footprinting, fluorescence labeling, and photo-cross-linking, we detected the formation of DNA:RNA hybrid G-quadruplexes (HQs) of two G-quartets during the transcription of DNA duplexes. These HQs have a lifetime on the minute scale and are stabilized by a stabilizing ligand. They are far shorter-lived than the HQs of three G-quartets, which last for hours. The occurrence of putative formation motifs of such HQs shows a transcription-dependent strand-biased selection, thus supporting their formation and function in genomes. They are present in almost all human genes in large amounts. We speculate that the two-G-quartet HQs may be a distinct type of G-quadruplexes that may play a role in timely responsive processes and for purposes of fine-tuning.},
	number = {48},
	journal = {Angewandte Chemie International Edition},
	author = {Xiao, Shan and Zhang, Jia-Yu and Wu, Jun and Wu, Ren-Yi and Xia, Ye and Zheng, Ke-Wei and Hao, Yu-Hua and Zhou, Xiang and Tan, Zheng},
	month = nov,
	year = {2014},
	pmid = {25267250},
	keywords = {\#nosource},
	pages = {13110--13114},
}

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