Formation and properties of hairpin and tetraplex structures of guanine-rich regulatory sequences of muscle-specific genes. Yafe, A., Etzioni, S., Weisman-Shomer, P., & Fry, M. Nucleic acids research, 33(9):2887–900, January, 2005. ![Formation and properties of hairpin and tetraplex structures of guanine-rich regulatory sequences of muscle-specific genes. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Clustered guanine residues in DNA readily generate hairpin or a variety of tetrahelical structures. The myogenic determination protein MyoD was reported to bind to a tetrahelical structure of guanine-rich enhancer sequence of muscle creatine kinase (MCK) more tightly than to its target E-box motif [K. Walsh and A. Gualberto (1992) J. Biol. Chem., 267, 13714-13718], suggesting that tetraplex structures of regulatory sequences of muscle-specific genes could contribute to transcriptional regulation. In the current study we show that promoter or enhancer sequences of various muscle-specific genes display a disproportionately high incidence of guanine clusters. The sequences derived from the guanine-rich promoter or enhancer regions of three muscle-specific genes, human sarcomeric mitochondrial creatine kinase (sMtCK), mouse MCK and alpha7 integrin formed diverse secondary structures. The sMtCK sequence folded into a hairpin structure; the alpha7 integrin oligonucleotide generated a unimolecular tetraplex; and sequences from all three genes associated to generate bimolecular tetraplexes. Furthermore, two neighboring non-contiguous guanine-rich tracts in the alpha7 integrin promoter region also paired to form a tetraplex structure. We also show that homodimeric MyoD bound bimolecular tetraplex structures of muscle-specific regulatory sequences more efficiently than its target E-box motif. These results are consistent with a role of tetrahelical structures of DNA in the regulation of muscle-specific gene expression.
@article{Yafe2005,
title = {Formation and properties of hairpin and tetraplex structures of guanine-rich regulatory sequences of muscle-specific genes.},
volume = {33},
issn = {1362-4962},
url = {http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1133794&tool=pmcentrez&rendertype=abstract},
doi = {10.1093/nar/gki606},
abstract = {Clustered guanine residues in DNA readily generate hairpin or a variety of tetrahelical structures. The myogenic determination protein MyoD was reported to bind to a tetrahelical structure of guanine-rich enhancer sequence of muscle creatine kinase (MCK) more tightly than to its target E-box motif [K. Walsh and A. Gualberto (1992) J. Biol. Chem., 267, 13714-13718], suggesting that tetraplex structures of regulatory sequences of muscle-specific genes could contribute to transcriptional regulation. In the current study we show that promoter or enhancer sequences of various muscle-specific genes display a disproportionately high incidence of guanine clusters. The sequences derived from the guanine-rich promoter or enhancer regions of three muscle-specific genes, human sarcomeric mitochondrial creatine kinase (sMtCK), mouse MCK and alpha7 integrin formed diverse secondary structures. The sMtCK sequence folded into a hairpin structure; the alpha7 integrin oligonucleotide generated a unimolecular tetraplex; and sequences from all three genes associated to generate bimolecular tetraplexes. Furthermore, two neighboring non-contiguous guanine-rich tracts in the alpha7 integrin promoter region also paired to form a tetraplex structure. We also show that homodimeric MyoD bound bimolecular tetraplex structures of muscle-specific regulatory sequences more efficiently than its target E-box motif. These results are consistent with a role of tetrahelical structures of DNA in the regulation of muscle-specific gene expression.},
number = {9},
journal = {Nucleic acids research},
author = {Yafe, Anat and Etzioni, Shulamit and Weisman-Shomer, Pnina and Fry, Michael},
month = jan,
year = {2005},
pmid = {15908587},
keywords = {\#nosource, Antigens, Base Sequence, CD, CD: genetics, Creatine Kinase, Creatine Kinase: genetics, DNA, DNA: chemistry, E-Box Elements, Enhancer Elements, G-Quadruplexes, Genetic, Guanine, Guanine: analysis, Integrin alpha Chains, Integrin alpha Chains: genetics, Isoenzymes, Isoenzymes: genetics, MM Form, Mitochondrial Form, Molecular Sequence Data, Muscles, Muscles: metabolism, MyoD Protein, MyoD Protein: metabolism, Nucleic Acid Conformation, Porphyrins, Porphyrins: chemistry, Promoter Regions, Temperature},
pages = {2887--900},
}
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Chem., 267, 13714-13718], suggesting that tetraplex structures of regulatory sequences of muscle-specific genes could contribute to transcriptional regulation. In the current study we show that promoter or enhancer sequences of various muscle-specific genes display a disproportionately high incidence of guanine clusters. The sequences derived from the guanine-rich promoter or enhancer regions of three muscle-specific genes, human sarcomeric mitochondrial creatine kinase (sMtCK), mouse MCK and alpha7 integrin formed diverse secondary structures. The sMtCK sequence folded into a hairpin structure; the alpha7 integrin oligonucleotide generated a unimolecular tetraplex; and sequences from all three genes associated to generate bimolecular tetraplexes. Furthermore, two neighboring non-contiguous guanine-rich tracts in the alpha7 integrin promoter region also paired to form a tetraplex structure. 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