Chapter 3 - Temnopleurus as an emerging echinoderm model

Chapter 3 - Temnopleurus as an emerging echinoderm model. Yaguchi, S. In Foltz, K. R. & Hamdoun, A., editors, Methods in Cell Biology, volume 150, of Echinoderms, Part A, pages 71–79. Academic Press, January, 2019.

Paper doi abstract bibtex

Sea urchins have played important roles in cell and developmental biology. They have the potential to be even more useful as models if the ability to create transgenic animals and maintain genetic lines are developed. Here, I describe the methods to produce next-generation lines using a newly introduced sea urchin model, Temnopleurus reevesii, in the laboratory. The embryos of T. reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry. I describe how to grow and culture the embryos/larvae/juveniles/adults of T. reevesii to address the challenge of establishing inbred strains followed by introducing genetics into this species in the future.

@incollection{yaguchi_chapter_2019,
	series = {Echinoderms, {Part} {A}},
	title = {Chapter 3 - {Temnopleurus} as an emerging echinoderm model},
	volume = {150},
	url = {http://www.sciencedirect.com/science/article/pii/S0091679X18301249},
	abstract = {Sea urchins have played important roles in cell and developmental biology. They have the potential to be even more useful as models if the ability to create transgenic animals and maintain genetic lines are developed. Here, I describe the methods to produce next-generation lines using a newly introduced sea urchin model, Temnopleurus reevesii, in the laboratory. The embryos of T. reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry. I describe how to grow and culture the embryos/larvae/juveniles/adults of T. reevesii to address the challenge of establishing inbred strains followed by introducing genetics into this species in the future.},
	urldate = {2019-05-13},
	booktitle = {Methods in {Cell} {Biology}},
	publisher = {Academic Press},
	author = {Yaguchi, Shunsuke},
	editor = {Foltz, Kathy R. and Hamdoun, Amro},
	month = jan,
	year = {2019},
	doi = {10.1016/bs.mcb.2018.09.001},
	keywords = {Yaguchi S},
	pages = {71--79}
}

Downloads: 0

{"_id":"bZzteTc2hnbSuk9so","bibbaseid":"yaguchi-chapter3temnopleurusasanemergingechinodermmodel-2019","authorIDs":[],"author_short":["Yaguchi, S."],"bibdata":{"bibtype":"incollection","type":"incollection","series":"Echinoderms, Part A","title":"Chapter 3 - Temnopleurus as an emerging echinoderm model","volume":"150","url":"http://www.sciencedirect.com/science/article/pii/S0091679X18301249","abstract":"Sea urchins have played important roles in cell and developmental biology. They have the potential to be even more useful as models if the ability to create transgenic animals and maintain genetic lines are developed. Here, I describe the methods to produce next-generation lines using a newly introduced sea urchin model, Temnopleurus reevesii, in the laboratory. The embryos of T. reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry. I describe how to grow and culture the embryos/larvae/juveniles/adults of T. reevesii to address the challenge of establishing inbred strains followed by introducing genetics into this species in the future.","urldate":"2019-05-13","booktitle":"Methods in Cell Biology","publisher":"Academic Press","author":[{"propositions":[],"lastnames":["Yaguchi"],"firstnames":["Shunsuke"],"suffixes":[]}],"editor":[{"propositions":[],"lastnames":["Foltz"],"firstnames":["Kathy","R."],"suffixes":[]},{"propositions":[],"lastnames":["Hamdoun"],"firstnames":["Amro"],"suffixes":[]}],"month":"January","year":"2019","doi":"10.1016/bs.mcb.2018.09.001","keywords":"Yaguchi S","pages":"71–79","bibtex":"@incollection{yaguchi_chapter_2019,\n\tseries = {Echinoderms, {Part} {A}},\n\ttitle = {Chapter 3 - {Temnopleurus} as an emerging echinoderm model},\n\tvolume = {150},\n\turl = {http://www.sciencedirect.com/science/article/pii/S0091679X18301249},\n\tabstract = {Sea urchins have played important roles in cell and developmental biology. They have the potential to be even more useful as models if the ability to create transgenic animals and maintain genetic lines are developed. Here, I describe the methods to produce next-generation lines using a newly introduced sea urchin model, Temnopleurus reevesii, in the laboratory. The embryos of T. reevesii have wide range of temperature tolerance between 15°C to 30°C and have high transparency, which can be a strong point in live-imaging and fluorescent immunohistochemistry. I describe how to grow and culture the embryos/larvae/juveniles/adults of T. reevesii to address the challenge of establishing inbred strains followed by introducing genetics into this species in the future.},\n\turldate = {2019-05-13},\n\tbooktitle = {Methods in {Cell} {Biology}},\n\tpublisher = {Academic Press},\n\tauthor = {Yaguchi, Shunsuke},\n\teditor = {Foltz, Kathy R. and Hamdoun, Amro},\n\tmonth = jan,\n\tyear = {2019},\n\tdoi = {10.1016/bs.mcb.2018.09.001},\n\tkeywords = {Yaguchi S},\n\tpages = {71--79}\n}\n\n","author_short":["Yaguchi, S."],"editor_short":["Foltz, K. R.","Hamdoun, A."],"key":"yaguchi_chapter_2019","id":"yaguchi_chapter_2019","bibbaseid":"yaguchi-chapter3temnopleurusasanemergingechinodermmodel-2019","role":"author","urls":{"Paper":"http://www.sciencedirect.com/science/article/pii/S0091679X18301249"},"keyword":["Yaguchi S"],"downloads":0,"html":"","metadata":{"authorlinks":{}}},"bibtype":"incollection","biburl":"http://www.shimoda.tsukuba.ac.jp/smrcbibtext.bib","creationDate":"2020-02-19T00:58:57.224Z","downloads":0,"keywords":["yaguchi s"],"search_terms":["chapter","temnopleurus","emerging","echinoderm","model","yaguchi"],"title":"Chapter 3 - Temnopleurus as an emerging echinoderm model","year":2019,"dataSources":["FmNRw89Cc4gQcNKAj"]}