Ubiquitous presence in mammalian cells of enzymatic activity specifically cleaving 8-hydroxyguanine-containing DNA. Yamamoto, F., Kasai, H., Bessho, T., Chung, M. H., Inoue, H., Ohtsuka, E., Hori, T., & Nishimura, S. Japanese Journal of Cancer Research: Gann, 83(4):351–357, April, 1992. doi abstract bibtex Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8-hydroxyguanine (oh8Gua) residues in various mammalian cells. To detect this activity, we used a synthetic double-stranded DNA containing a single oh8Gua at a defined position as the substrate, and analyzed the products of enzymatic digestion by polyacrylamide gel electrophoresis. Two cleavage sites near the oh8Gua residue were detected with partially purified fractions from cow brain and rat liver, and also with preparations from all mammalian tissues examined. These results suggest that enzymatic activity for the removal of oh8Gua from DNA is widely distributed in mammalian cells.
@article{yamamoto_ubiquitous_1992,
title = {Ubiquitous presence in mammalian cells of enzymatic activity specifically cleaving 8-hydroxyguanine-containing {DNA}},
volume = {83},
issn = {0910-5050},
doi = {10.1111/j.1349-7006.1992.tb00114.x},
abstract = {Here we report the finding of enzymatic activity that specifically cleaves DNA containing 8-hydroxyguanine (oh8Gua) residues in various mammalian cells. To detect this activity, we used a synthetic double-stranded DNA containing a single oh8Gua at a defined position as the substrate, and analyzed the products of enzymatic digestion by polyacrylamide gel electrophoresis. Two cleavage sites near the oh8Gua residue were detected with partially purified fractions from cow brain and rat liver, and also with preparations from all mammalian tissues examined. These results suggest that enzymatic activity for the removal of oh8Gua from DNA is widely distributed in mammalian cells.},
language = {eng},
number = {4},
journal = {Japanese Journal of Cancer Research: Gann},
author = {Yamamoto, F. and Kasai, H. and Bessho, T. and Chung, M. H. and Inoue, H. and Ohtsuka, E. and Hori, T. and Nishimura, S.},
month = apr,
year = {1992},
pmid = {1506269},
pmcid = {PMC5918833},
keywords = {Animals, Base Sequence, Brain, Cattle, DNA, DNA Repair, DNA-Formamidopyrimidine Glycosylase, Escherichia coli, Escherichia coli Proteins, Liver, Mammals, Molecular Sequence Data, N-Glycosyl Hydrolases, Oligodeoxyribonucleotides, Organ Specificity, Rats, Species Specificity, Substrate Specificity},
pages = {351--357},
}
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