Proteome phenotyping of Delta relA mutants in Enterococcus faecalis V583. Yan, X., Budin-Verneuil, A., Auffray, Y., & Pichereau, V. Canadian journal of microbiology, 60(8):525--531, 2014.
Paper doi abstract bibtex The (p) ppGpp synthetase RelA contributes to stress adaptation and virulence in Enterococcus faecalis V583. A 2-dimensional electrophoresis proteomic analysis of 2 relA mutants, i.e., Delta relA carrying a complete deletion of the relA gene, and Delta relAsp that is deleted from only its 3' extremity, showed that 31 proteins were deregulated in 1 or both of these mutants. Mass spectrometry identification of these proteins showed that 10 are related to translation, including 5 ribosomal proteins, 3 proteins involved in translation elongation, and 2 proteins in tRNA synthesis; 14 proteins are involved in diverse metabolisms and biosynthesis (8 in sugar and energy metabolisms, 2 in fatty acid biosynthesis, 2 in amino acid biosynthesis, and 2 in nucleotide metabolism). Five proteins were relevant to the adaptation to different environmental stresses, i.e., SodA and a Dps family protein, 2 cold-shock domain proteins, and Ef1744, which is a general stress protein that plays an important role in the response to ethanol stress. The potential role of these proteins in the development of stress phenotypes associated with these mutations is discussed.
@article{yan_proteome_2014,
title = {Proteome phenotyping of {Delta} {relA} mutants in {Enterococcus} faecalis {V}583},
volume = {60},
issn = {0008-4166},
url = {http://www.nrcresearchpress.com/doi/abs/10.1139/cjm-2014-0254},
doi = {10.1139/cjm-2014-0254},
abstract = {The (p) ppGpp synthetase RelA contributes to stress adaptation and virulence in Enterococcus faecalis V583. A 2-dimensional electrophoresis proteomic analysis of 2 relA mutants, i.e., Delta relA carrying a complete deletion of the relA gene, and Delta relAsp that is deleted from only its 3' extremity, showed that 31 proteins were deregulated in 1 or both of these mutants. Mass spectrometry identification of these proteins showed that 10 are related to translation, including 5 ribosomal proteins, 3 proteins involved in translation elongation, and 2 proteins in tRNA synthesis; 14 proteins are involved in diverse metabolisms and biosynthesis (8 in sugar and energy metabolisms, 2 in fatty acid biosynthesis, 2 in amino acid biosynthesis, and 2 in nucleotide metabolism). Five proteins were relevant to the adaptation to different environmental stresses, i.e., SodA and a Dps family protein, 2 cold-shock domain proteins, and Ef1744, which is a general stress protein that plays an important role in the response to ethanol stress. The potential role of these proteins in the development of stress phenotypes associated with these mutations is discussed.},
number = {8},
urldate = {2015-04-20TZ},
journal = {Canadian journal of microbiology},
author = {Yan, Xue and Budin-Verneuil, Aurélie and Auffray, Yanick and Pichereau, Vianney},
year = {2014},
keywords = {ACL, E1, WOS, general stress protein, guanine nucleotides, proteome, relA mutants},
pages = {525--531}
}
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