Hepatitis C virus core proteins derived from different quasispecies of genotype 1b inhibit the growth of Chang liver cells. Yan, X. B., Mei, L., Feng, X., Wan, M. R., Chen, Z., Pavio, N., & Brechot, C. World J Gastroenterol, 14(18):2877–81, May, 2008.
abstract   bibtex   
AIM: To investigate the influence of different quasispecies of hepatitis C virus (HCV) genotype 1b core protein on growth of Chang liver cells. METHODS: Three eukaryotic expression plasmids (pEGFP-N1/core) that contained different quasispecies truncated core proteins of HCV genotype 1b were constructed. These were derived from tumor (T) and non-tumor (NT) tissues of a patient infected with HCV and C191 (HCV-J6). The core protein expression plasmids were transiently transfected into Chang liver cells. At different times, the cell cycle and apoptosis was assayed by flow cytometry, and cell proliferation was assayed by methyl thiazolyl tetrazolium (MTT) assay. RESULTS: The proportion of S-phase Chang liver cells transfected with pEGFP-N1/core was significantly lower than that of cells transfected with blank plasmid at three different times after transfection (all P \textless 0.05). The proliferation ratio of cells transfected with pEGFP-N1/core was significantly lower than that of cells transfected with blank plasmid. Among three different quasispecies, T, NT and C191 core expression cells, there was no significant difference in the proportion of S- and G0/G1-phase cells. The percentage of apoptotic cells was highest for T (T \textgreater NT \textgreater C191), and apoptosis was increased in cells transfected with pEGFP-N1/core as the transfection time increased (72 h \textgreater 48 h \textgreater 24 h). CONCLUSION: These results suggest that HCV genotype 1b core protein induces apoptosis, and inhibits cell-cycle progression and proliferation of Chang liver cells. Different quasispecies core proteins of HCV genotype 1b might have some differences in the pathogenesis of HCV persistent infection and hepatocellular carcinoma.
@article{yan_hepatitis_2008,
	title = {Hepatitis {C} virus core proteins derived from different quasispecies of genotype 1b inhibit the growth of {Chang} liver cells},
	volume = {14},
	issn = {1007-9327 (PRINT); 1007-9327 (LINKING)},
	shorttitle = {Hepatitis {C} virus core proteins derived from different quasispecies of genotype 1b inhibit the growth of {Chang} liver cells},
	abstract = {AIM: To investigate the influence of different quasispecies of hepatitis C virus (HCV) genotype 1b core protein on growth of Chang liver cells. METHODS: Three eukaryotic expression plasmids (pEGFP-N1/core) that contained different quasispecies truncated core proteins of HCV genotype 1b were constructed. These were derived from tumor (T) and non-tumor (NT) tissues of a patient infected with HCV and C191 (HCV-J6). The core protein expression plasmids were transiently transfected into Chang liver cells. At different times, the cell cycle and apoptosis was assayed by flow cytometry, and cell proliferation was assayed by methyl thiazolyl tetrazolium (MTT) assay. RESULTS: The proportion of S-phase Chang liver cells transfected with pEGFP-N1/core was significantly lower than that of cells transfected with blank plasmid at three different times after transfection (all P {\textless} 0.05). The proliferation ratio of cells transfected with pEGFP-N1/core was significantly lower than that of cells transfected with blank plasmid. Among three different quasispecies, T, NT and C191 core expression cells, there was no significant difference in the proportion of S- and G0/G1-phase cells. The percentage of apoptotic cells was highest for T (T {\textgreater} NT {\textgreater} C191), and apoptosis was increased in cells transfected with pEGFP-N1/core as the transfection time increased (72 h {\textgreater} 48 h {\textgreater} 24 h). CONCLUSION: These results suggest that HCV genotype 1b core protein induces apoptosis, and inhibits cell-cycle progression and proliferation of Chang liver cells. Different quasispecies core proteins of HCV genotype 1b might have some differences in the pathogenesis of HCV persistent infection and hepatocellular carcinoma.},
	number = {18},
	journal = {World J Gastroenterol},
	author = {Yan, X. B. and Mei, L. and Feng, X. and Wan, M. R. and Chen, Z. and Pavio, N. and Brechot, C.},
	month = may,
	year = {2008},
	keywords = {Apoptosis/drug, Cell, Core, Cycle/drug, Genotype, Hepacivirus/, Humans, Liver/, Plasmids, Proliferation/, Proteins/, Transfection, Viral, chemistry/genetics, cytology/drug, drug, effects, pharmacology},
	pages = {2877--81}
}

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