Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing. Yang, X., Liu, D., Lu, P., Zhang, Y., & Yu, C. The Analyst, 135(8):2074–8, August, 2010. ![Nucleic acid G-quadruplex based label-free fluorescence turn-on potassium selective sensing. [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex We report a label-free fluorescence turn-on approach for the selective sensing of potassium. A properly selected G-rich oligonucleotide (oligo-Y) folded into stable quadruplex structure when mixed with potassium in an aqueous solution. Single-stranded nucleic acid specific nuclease was subsequently added. Since an oligonucleotide in quadruplex structure is markedly more resistant to nuclease digestion than in its random coil conformation, oligo-Y digestion by nuclease was considerably slow. On the other hand, oligo-Y mixed with other common mono- or divalent ions was completely digested in 5 min under our experimental conditions because no quadruplex or less stable quadruplex was formed. Oligo-Y in potassium was subsequently mixed with a positively charged pyrene probe. Electrostatic interactions between oligo-Y (a polyanion) and the probe induced aggregation of the probe, which in turn induced strong pyrene excimer emission. The intensity of the induced excimer emission was directly proportional to the amount of potassium added. Our method shows good sensitivity, and good selectivity against other common interference ions.
@article{Yang2010,
title = {Nucleic acid {G}-quadruplex based label-free fluorescence turn-on potassium selective sensing.},
volume = {135},
issn = {1364-5528},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20585688},
doi = {10.1039/c0an00106f},
abstract = {We report a label-free fluorescence turn-on approach for the selective sensing of potassium. A properly selected G-rich oligonucleotide (oligo-Y) folded into stable quadruplex structure when mixed with potassium in an aqueous solution. Single-stranded nucleic acid specific nuclease was subsequently added. Since an oligonucleotide in quadruplex structure is markedly more resistant to nuclease digestion than in its random coil conformation, oligo-Y digestion by nuclease was considerably slow. On the other hand, oligo-Y mixed with other common mono- or divalent ions was completely digested in 5 min under our experimental conditions because no quadruplex or less stable quadruplex was formed. Oligo-Y in potassium was subsequently mixed with a positively charged pyrene probe. Electrostatic interactions between oligo-Y (a polyanion) and the probe induced aggregation of the probe, which in turn induced strong pyrene excimer emission. The intensity of the induced excimer emission was directly proportional to the amount of potassium added. Our method shows good sensitivity, and good selectivity against other common interference ions.},
number = {8},
journal = {The Analyst},
author = {Yang, Xiangyu and Liu, Dan and Lu, Ping and Zhang, Yujing and Yu, Cong},
month = aug,
year = {2010},
pmid = {20585688},
keywords = {\#nosource, Fluorescence, G-Quadruplexes, Molecular Structure, Nucleic Acids, Nucleic Acids: chemistry, Potassium, Potassium: analysis, Single-Strand Specific DNA and RNA Endonucleases, Single-Strand Specific DNA and RNA Endonucleases:},
pages = {2074--8},
}
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On the other hand, oligo-Y mixed with other common mono- or divalent ions was completely digested in 5 min under our experimental conditions because no quadruplex or less stable quadruplex was formed. Oligo-Y in potassium was subsequently mixed with a positively charged pyrene probe. Electrostatic interactions between oligo-Y (a polyanion) and the probe induced aggregation of the probe, which in turn induced strong pyrene excimer emission. The intensity of the induced excimer emission was directly proportional to the amount of potassium added. 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