The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of Malania oleifera. Yang, T., Zhang, R., Tian, X., Yao, G., Shen, Y., Wang, S., Mao, J., Li, G., Liu, A., Sun, W., & Ma, Y. Scientific Data, 10(1):298, May, 2023. Number: 1 Publisher: Nature Publishing Group![The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of Malania oleifera [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Nervonic acid (C24:1 Δ15, NA) is a very long-chain monounsaturated fatty acid, a clinically indispensable resource in maintaining the brain and nerve cells development and regeneration. Till now, NA has been found in 38 plant species, among which the garlic-fruit tree (Malania oleifera) has been evaluated to be the best candidate for NA production. Here, we generated a high-quality chromosome-scale assembly of M. oleifera employing PacBio long-read, short-read Illumina as well as Hi-C sequencing data. The genome assembly consisted of 1.5 Gb with a contig N50 of ~4.9 Mb and a scaffold N50 of ~112.6 Mb. ~98.2% of the assembly was anchored into 13 pseudo-chromosomes. It contains ~1123 Mb repeat sequences, and 27,638 protein-coding genes, 568 tRNAs, 230 rRNAs and 352 other non-coding RNAs. Additionally, we documented candidate genes involved in NA biosynthesis including 20 KCSs, 4 KCRs, 1 HCD and 1 ECR, and profiled the expression patterns of these genes in developing seeds. The high-quality assembly of the genome provides insights into the genome evolution of the M. oleifera genome and candidate genes involved in NA biosynthesis in the seeds of this important woody tree.
@article{yang_chromosome-level_2023,
title = {The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of {Malania} oleifera},
volume = {10},
copyright = {2023 The Author(s)},
issn = {2052-4463},
url = {https://www.nature.com/articles/s41597-023-02218-8},
doi = {10.1038/s41597-023-02218-8},
abstract = {Nervonic acid (C24:1 Δ15, NA) is a very long-chain monounsaturated fatty acid, a clinically indispensable resource in maintaining the brain and nerve cells development and regeneration. Till now, NA has been found in 38 plant species, among which the garlic-fruit tree (Malania oleifera) has been evaluated to be the best candidate for NA production. Here, we generated a high-quality chromosome-scale assembly of M. oleifera employing PacBio long-read, short-read Illumina as well as Hi-C sequencing data. The genome assembly consisted of 1.5 Gb with a contig N50 of {\textasciitilde}4.9 Mb and a scaffold N50 of {\textasciitilde}112.6 Mb. {\textasciitilde}98.2\% of the assembly was anchored into 13 pseudo-chromosomes. It contains {\textasciitilde}1123 Mb repeat sequences, and 27,638 protein-coding genes, 568 tRNAs, 230 rRNAs and 352 other non-coding RNAs. Additionally, we documented candidate genes involved in NA biosynthesis including 20 KCSs, 4 KCRs, 1 HCD and 1 ECR, and profiled the expression patterns of these genes in developing seeds. The high-quality assembly of the genome provides insights into the genome evolution of the M. oleifera genome and candidate genes involved in NA biosynthesis in the seeds of this important woody tree.},
language = {en},
number = {1},
urldate = {2023-05-26},
journal = {Scientific Data},
author = {Yang, Tianquan and Zhang, Rengang and Tian, Xiaoling and Yao, Gang and Shen, Yuanting and Wang, Sihai and Mao, Jianfeng and Li, Guangyuan and Liu, Aizhong and Sun, Weibang and Ma, Yongpeng},
month = may,
year = {2023},
note = {Number: 1
Publisher: Nature Publishing Group},
keywords = {Genome, Genomics},
pages = {298},
}
Downloads: 0
{"_id":"s4ey8KQF24QF77NRR","bibbaseid":"yang-zhang-tian-yao-shen-wang-mao-li-etal-thechromosomelevelgenomeassemblyandgenesinvolvedinbiosynthesisofnervonicacidofmalaniaoleifera-2023","author_short":["Yang, T.","Zhang, R.","Tian, X.","Yao, G.","Shen, Y.","Wang, S.","Mao, J.","Li, G.","Liu, A.","Sun, W.","Ma, Y."],"bibdata":{"bibtype":"article","type":"article","title":"The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of Malania oleifera","volume":"10","copyright":"2023 The Author(s)","issn":"2052-4463","url":"https://www.nature.com/articles/s41597-023-02218-8","doi":"10.1038/s41597-023-02218-8","abstract":"Nervonic acid (C24:1 Δ15, NA) is a very long-chain monounsaturated fatty acid, a clinically indispensable resource in maintaining the brain and nerve cells development and regeneration. Till now, NA has been found in 38 plant species, among which the garlic-fruit tree (Malania oleifera) has been evaluated to be the best candidate for NA production. Here, we generated a high-quality chromosome-scale assembly of M. oleifera employing PacBio long-read, short-read Illumina as well as Hi-C sequencing data. The genome assembly consisted of 1.5 Gb with a contig N50 of ~4.9 Mb and a scaffold N50 of ~112.6 Mb. ~98.2% of the assembly was anchored into 13 pseudo-chromosomes. It contains ~1123 Mb repeat sequences, and 27,638 protein-coding genes, 568 tRNAs, 230 rRNAs and 352 other non-coding RNAs. Additionally, we documented candidate genes involved in NA biosynthesis including 20 KCSs, 4 KCRs, 1 HCD and 1 ECR, and profiled the expression patterns of these genes in developing seeds. The high-quality assembly of the genome provides insights into the genome evolution of the M. oleifera genome and candidate genes involved in NA biosynthesis in the seeds of this important woody tree.","language":"en","number":"1","urldate":"2023-05-26","journal":"Scientific Data","author":[{"propositions":[],"lastnames":["Yang"],"firstnames":["Tianquan"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Rengang"],"suffixes":[]},{"propositions":[],"lastnames":["Tian"],"firstnames":["Xiaoling"],"suffixes":[]},{"propositions":[],"lastnames":["Yao"],"firstnames":["Gang"],"suffixes":[]},{"propositions":[],"lastnames":["Shen"],"firstnames":["Yuanting"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Sihai"],"suffixes":[]},{"propositions":[],"lastnames":["Mao"],"firstnames":["Jianfeng"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Guangyuan"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Aizhong"],"suffixes":[]},{"propositions":[],"lastnames":["Sun"],"firstnames":["Weibang"],"suffixes":[]},{"propositions":[],"lastnames":["Ma"],"firstnames":["Yongpeng"],"suffixes":[]}],"month":"May","year":"2023","note":"Number: 1 Publisher: Nature Publishing Group","keywords":"Genome, Genomics","pages":"298","bibtex":"@article{yang_chromosome-level_2023,\n\ttitle = {The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of {Malania} oleifera},\n\tvolume = {10},\n\tcopyright = {2023 The Author(s)},\n\tissn = {2052-4463},\n\turl = {https://www.nature.com/articles/s41597-023-02218-8},\n\tdoi = {10.1038/s41597-023-02218-8},\n\tabstract = {Nervonic acid (C24:1 Δ15, NA) is a very long-chain monounsaturated fatty acid, a clinically indispensable resource in maintaining the brain and nerve cells development and regeneration. Till now, NA has been found in 38 plant species, among which the garlic-fruit tree (Malania oleifera) has been evaluated to be the best candidate for NA production. Here, we generated a high-quality chromosome-scale assembly of M. oleifera employing PacBio long-read, short-read Illumina as well as Hi-C sequencing data. The genome assembly consisted of 1.5 Gb with a contig N50 of {\\textasciitilde}4.9 Mb and a scaffold N50 of {\\textasciitilde}112.6 Mb. {\\textasciitilde}98.2\\% of the assembly was anchored into 13 pseudo-chromosomes. It contains {\\textasciitilde}1123 Mb repeat sequences, and 27,638 protein-coding genes, 568 tRNAs, 230 rRNAs and 352 other non-coding RNAs. Additionally, we documented candidate genes involved in NA biosynthesis including 20 KCSs, 4 KCRs, 1 HCD and 1 ECR, and profiled the expression patterns of these genes in developing seeds. The high-quality assembly of the genome provides insights into the genome evolution of the M. oleifera genome and candidate genes involved in NA biosynthesis in the seeds of this important woody tree.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2023-05-26},\n\tjournal = {Scientific Data},\n\tauthor = {Yang, Tianquan and Zhang, Rengang and Tian, Xiaoling and Yao, Gang and Shen, Yuanting and Wang, Sihai and Mao, Jianfeng and Li, Guangyuan and Liu, Aizhong and Sun, Weibang and Ma, Yongpeng},\n\tmonth = may,\n\tyear = {2023},\n\tnote = {Number: 1\nPublisher: Nature Publishing Group},\n\tkeywords = {Genome, Genomics},\n\tpages = {298},\n}\n\n\n\n","author_short":["Yang, T.","Zhang, R.","Tian, X.","Yao, G.","Shen, Y.","Wang, S.","Mao, J.","Li, G.","Liu, A.","Sun, W.","Ma, Y."],"key":"yang_chromosome-level_2023","id":"yang_chromosome-level_2023","bibbaseid":"yang-zhang-tian-yao-shen-wang-mao-li-etal-thechromosomelevelgenomeassemblyandgenesinvolvedinbiosynthesisofnervonicacidofmalaniaoleifera-2023","role":"author","urls":{"Paper":"https://www.nature.com/articles/s41597-023-02218-8"},"keyword":["Genome","Genomics"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/zotero/upscpub","dataSources":["3zTPPmKj8BiTcpc6C","9cGcv2t8pRzC92kzs"],"keywords":["genome","genomics"],"search_terms":["chromosome","level","genome","assembly","genes","involved","biosynthesis","nervonic","acid","malania","oleifera","yang","zhang","tian","yao","shen","wang","mao","li","liu","sun","ma"],"title":"The chromosome-level genome assembly and genes involved in biosynthesis of nervonic acid of Malania oleifera","year":2023}