Enhancement of bacterial competitive fitness by apramycin resistance plasmids from non-pathogenic Escherichia coli. Yates, C. M., Shaw, D. J., Roe, A. J., Woolhouse, M. E. J., & Amyes, S. G. B. Biology letters, 2(3):463--465, September, 2006.
doi  abstract   bibtex   
The study of antibiotic resistance has in the past focused on organisms that are pathogenic to humans or animals. However, the development of resistance in commensal organisms is of concern because of possible transfer of resistance genes to zoonotic pathogens. Conjugative plasmids are genetic elements capable of such transfer and are traditionally thought to engender a fitness burden on host bacteria. In this study, conjugative apramycin resistance plasmids isolated from newborn calves were characterized. Calves were raised on a farm that had not used apramycin or related aminoglycoside antibiotics for at least 20 months prior to sampling. Of three apramycin resistance plasmids, one was capable of transfer at very high rates and two were found to confer fitness advantages on new Escherichia coli hosts. This is the first identification of natural plasmids isolated from commensal organisms that are able to confer a fitness advantage on a new host. This work indicates that reservoirs of antibiotic resistance genes in commensal organisms might not decrease if antibiotic usage is halted.
@article{yates_enhancement_2006,
	title = {Enhancement of bacterial competitive fitness by apramycin resistance plasmids from non-pathogenic {Escherichia} coli.},
	volume = {2},
	issn = {1744-9561 1744-9561},
	doi = {10.1098/rsbl.2006.0478},
	abstract = {The study of antibiotic resistance has in the past focused on organisms that are  pathogenic to humans or animals. However, the development of resistance in commensal organisms is of concern because of possible transfer of resistance genes to zoonotic pathogens. Conjugative plasmids are genetic elements capable of such transfer and are traditionally thought to engender a fitness burden on host  bacteria. In this study, conjugative apramycin resistance plasmids isolated from  newborn calves were characterized. Calves were raised on a farm that had not used apramycin or related aminoglycoside antibiotics for at least 20 months prior to sampling. Of three apramycin resistance plasmids, one was capable of transfer at  very high rates and two were found to confer fitness advantages on new Escherichia coli hosts. This is the first identification of natural plasmids isolated from commensal organisms that are able to confer a fitness advantage on  a new host. This work indicates that reservoirs of antibiotic resistance genes in commensal organisms might not decrease if antibiotic usage is halted.},
	language = {eng},
	number = {3},
	journal = {Biology letters},
	author = {Yates, C. M. and Shaw, D. J. and Roe, A. J. and Woolhouse, M. E. J. and Amyes, S. G. B.},
	month = sep,
	year = {2006},
	pmid = {17148431},
	pmcid = {PMC1686191},
	keywords = {*Drug Resistance, *Drug Resistance, Bacterial, Animals, Anti-Bacterial Agents/metabolism, Cattle, Conjugation, Genetic, Escherichia coli/*genetics/metabolism, Gene Transfer Techniques, Gene Transfer, Horizontal, Models, Genetic, Nebramycin/*analogs \& derivatives/pharmacology, Phenotype, Plasmids/metabolism},
	pages = {463--465}
}
Downloads: 0