Antimicrobial effect and membrane-active mechanism of tea polyphenols against Serratia marcescens. Yi, S., Wang, W., Bai, F., Zhu, J., Li, J., Li, X., Xu, Y., Sun, T., & He, Y. World Journal of Microbiology & Biotechnology, 30(2):451–460, February, 2014. WOS:000330829900011doi abstract bibtex In this study, we investigated the antimicrobial effect of tea polyphenols (TP) against Serratia marcescens and examined the related mechanism. Morphology changes of S. marcescens were first observed by transmission electron microscopy after treatment with TP, which indicated that the primary inhibition action of TP was to damage the bacterial cell membranes. The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. Taken together, our results first demonstrated that TP had a significant growth inhibition effect on S. marcescens through cell membrane damage.
@article{yi_antimicrobial_2014,
title = {Antimicrobial effect and membrane-active mechanism of tea polyphenols against {Serratia} marcescens},
volume = {30},
issn = {0959-3993},
doi = {10.1007/s11274-013-1464-4},
abstract = {In this study, we investigated the antimicrobial effect of tea polyphenols (TP) against Serratia marcescens and examined the related mechanism. Morphology changes of S. marcescens were first observed by transmission electron microscopy after treatment with TP, which indicated that the primary inhibition action of TP was to damage the bacterial cell membranes. The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. Taken together, our results first demonstrated that TP had a significant growth inhibition effect on S. marcescens through cell membrane damage.},
language = {English},
number = {2},
journal = {World Journal of Microbiology \& Biotechnology},
author = {Yi, Shumin and Wang, Wei and Bai, Fengling and Zhu, Junli and Li, Jianrong and Li, Xuepeng and Xu, Yongxia and Sun, Tong and He, Yutang},
month = feb,
year = {2014},
note = {WOS:000330829900011},
keywords = {Antioxidant, Membrane-active mechanism, Serratia marcescens, Tea polyphenols, bacillus-subtilis, bacteria, binding proteins, chaperones, coli, food, health, in-vivo, machines},
pages = {451--460},
}
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The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. Taken together, our results first demonstrated that TP had a significant growth inhibition effect on S. marcescens through cell membrane damage.","language":"English","number":"2","journal":"World Journal of Microbiology & Biotechnology","author":[{"propositions":[],"lastnames":["Yi"],"firstnames":["Shumin"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Wei"],"suffixes":[]},{"propositions":[],"lastnames":["Bai"],"firstnames":["Fengling"],"suffixes":[]},{"propositions":[],"lastnames":["Zhu"],"firstnames":["Junli"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Jianrong"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Xuepeng"],"suffixes":[]},{"propositions":[],"lastnames":["Xu"],"firstnames":["Yongxia"],"suffixes":[]},{"propositions":[],"lastnames":["Sun"],"firstnames":["Tong"],"suffixes":[]},{"propositions":[],"lastnames":["He"],"firstnames":["Yutang"],"suffixes":[]}],"month":"February","year":"2014","note":"WOS:000330829900011","keywords":"Antioxidant, Membrane-active mechanism, Serratia marcescens, Tea polyphenols, bacillus-subtilis, bacteria, binding proteins, chaperones, coli, food, health, in-vivo, machines","pages":"451–460","bibtex":"@article{yi_antimicrobial_2014,\n\ttitle = {Antimicrobial effect and membrane-active mechanism of tea polyphenols against {Serratia} marcescens},\n\tvolume = {30},\n\tissn = {0959-3993},\n\tdoi = {10.1007/s11274-013-1464-4},\n\tabstract = {In this study, we investigated the antimicrobial effect of tea polyphenols (TP) against Serratia marcescens and examined the related mechanism. Morphology changes of S. marcescens were first observed by transmission electron microscopy after treatment with TP, which indicated that the primary inhibition action of TP was to damage the bacterial cell membranes. The permeability of the outer and inner membrane of S. marcescens dramatically increased after TP treatment, which caused severe disruption of cell membrane, followed by the release of small cellular molecules. Furthermore, a proteomics approach based on two-dimensional gel electrophoresis and MALDI-TOF/TOF MS analysis was used to study the difference of membrane protein expression in the control and TP treatment S. marcescens. The results showed that the expression of some metabolism enzymes and chaperones in TP-treated S. marcescens significantly increased compared to the untreated group, which might result in the metabolic disorder of this bacteria. 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