Stimulation of ribosomal frameshifting by RNA G-quadruplex structures.

Stimulation of ribosomal frameshifting by RNA G-quadruplex structures. Yu, C., Teulade-Fichou, M., & Olsthoorn, R. C L Nucleic acids research, October, 2013.

Paper doi abstract bibtex

Guanine-rich sequences can fold into four-stranded structures of stacked guanine-tetrads, so-called G-quadruplexes (G4). These unique motifs have been extensively studied on the DNA level; however, exploration of the biological roles of G4s at the RNA level is just emerging. Here we show that G4 RNA when introduced within coding regions are capable of stimulating -1 ribosomal frameshifting (-1 FS) in vitro and in cultured cells. Systematic manipulation of the loop length between each G-tract revealed that the -1 FS efficiency positively correlates with G4 stability. Addition of a G4-stabilizing ligand, PhenDC3, resulted in higher -1 FS. Further, we demonstrated that the G4s can stimulate +1 FS and stop codon readthrough as well. These results suggest a potentially novel translational gene regulation mechanism mediated by G4 RNA.

@article{Yu2013,
	title = {Stimulation of ribosomal frameshifting by {RNA} {G}-quadruplex structures.},
	issn = {1362-4962},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/24178029},
	doi = {10.1093/nar/gkt1022},
	abstract = {Guanine-rich sequences can fold into four-stranded structures of stacked guanine-tetrads, so-called G-quadruplexes (G4). These unique motifs have been extensively studied on the DNA level; however, exploration of the biological roles of G4s at the RNA level is just emerging. Here we show that G4 RNA when introduced within coding regions are capable of stimulating -1 ribosomal frameshifting (-1 FS) in vitro and in cultured cells. Systematic manipulation of the loop length between each G-tract revealed that the -1 FS efficiency positively correlates with G4 stability. Addition of a G4-stabilizing ligand, PhenDC3, resulted in higher -1 FS. Further, we demonstrated that the G4s can stimulate +1 FS and stop codon readthrough as well. These results suggest a potentially novel translational gene regulation mechanism mediated by G4 RNA.},
	journal = {Nucleic acids research},
	author = {Yu, Chien-Hung and Teulade-Fichou, Marie-Paule and Olsthoorn, René C L},
	month = oct,
	year = {2013},
	pmid = {24178029},
	keywords = {\#nosource},
	pages = {1--6},
}

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