Incomplete radiofrequency ablation promotes the development of CD133+ cancer stem cells in hepatocellular carcinoma cell line HepG2 via inducing SOX9 expression. Yuan, C., Wang, Z., Liu, K., & Liu, D. Hepatobiliary & Pancreatic Diseases International, September, 2018. ![Incomplete radiofrequency ablation promotes the development of CD133+ cancer stem cells in hepatocellular carcinoma cell line HepG2 via inducing SOX9 expression [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Background Cancer stem cells (CSCs) accelerate the growth of hepatocellular carcinoma (HCC) residual after incomplete radiofrequency ablation (In-RFA). The present study aimed to detect the effects of In-RFA on stemness transcription factors (STFs) expression which are important for the production and function of CSCs, and to find which STFs promote HCC stemness after In-RFA. Methods HepG2 cells were used for in vitro and in vivo studies. Flow cytometry and sphere-formation assays were used to detect the level and function of CD133+CSCs in the models. PCR array and ELISA were applied to analyze the altered expression of 84 STFs in CD133+CSCs in two models. Specific lentiviral shRNA was used to knockdown STFs expression, followed by detecting In-RFA's effects on the levels and function of CD133+CSCs. Results In-RFA was identified to induce CD133+CSCs and increase their tumorigenesis ability in vitro and in vivo. The mRNA levels of 84 STFs in CD133+CSCs were detected by PCR array, showing that 15 and 22 STFs were up-regulated in two models, respectively. Meanwhile, the mRNA levels of seven common STFs were up-regulated in both models. ELISA assay demonstrated that only the protein of sex determining region Y-box 9 (SOX9) was up-regulated in both models, the protein levels of the other 6 common STFs did not increase in both models. Finally, SOX9 was identified to play an important role in inducing, maintaining stemness and promoting tumorigenesis ability of CD133+CSCs in both models. Conclusion In-RFA-induced SOX9 stimulates CD133+CSCs proliferation and increases their tumorigenesis ability, suggesting that SOX9 may be a good target for HCC treatment.
@article{yuan_incomplete_2018,
title = {Incomplete radiofrequency ablation promotes the development of {CD133}+ cancer stem cells in hepatocellular carcinoma cell line {HepG2} via inducing {SOX9} expression},
issn = {1499-3872},
url = {http://www.sciencedirect.com/science/article/pii/S1499387218302029},
doi = {10.1016/j.hbpd.2018.09.012},
abstract = {Background
Cancer stem cells (CSCs) accelerate the growth of hepatocellular carcinoma (HCC) residual after incomplete radiofrequency ablation (In-RFA). The present study aimed to detect the effects of In-RFA on stemness transcription factors (STFs) expression which are important for the production and function of CSCs, and to find which STFs promote HCC stemness after In-RFA.
Methods
HepG2 cells were used for in vitro and in vivo studies. Flow cytometry and sphere-formation assays were used to detect the level and function of CD133+CSCs in the models. PCR array and ELISA were applied to analyze the altered expression of 84 STFs in CD133+CSCs in two models. Specific lentiviral shRNA was used to knockdown STFs expression, followed by detecting In-RFA's effects on the levels and function of CD133+CSCs.
Results
In-RFA was identified to induce CD133+CSCs and increase their tumorigenesis ability in vitro and in vivo. The mRNA levels of 84 STFs in CD133+CSCs were detected by PCR array, showing that 15 and 22 STFs were up-regulated in two models, respectively. Meanwhile, the mRNA levels of seven common STFs were up-regulated in both models. ELISA assay demonstrated that only the protein of sex determining region Y-box 9 (SOX9) was up-regulated in both models, the protein levels of the other 6 common STFs did not increase in both models. Finally, SOX9 was identified to play an important role in inducing, maintaining stemness and promoting tumorigenesis ability of CD133+CSCs in both models.
Conclusion
In-RFA-induced SOX9 stimulates CD133+CSCs proliferation and increases their tumorigenesis ability, suggesting that SOX9 may be a good target for HCC treatment.},
urldate = {2018-09-29},
journal = {Hepatobiliary \& Pancreatic Diseases International},
author = {Yuan, Chun-Wang and Wang, Zhen-Chang and Liu, Kai and Liu, Dong-Jie},
month = sep,
year = {2018},
keywords = {Cancer stem cells, HepG2, Hepatocellular carcinoma, Radiofrequency ablation},
}
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{"_id":"iZEkp5sfuztgPTikJ","bibbaseid":"yuan-wang-liu-liu-incompleteradiofrequencyablationpromotesthedevelopmentofcd133cancerstemcellsinhepatocellularcarcinomacelllinehepg2viainducingsox9expression-2018","downloads":0,"creationDate":"2019-03-08T19:02:46.051Z","title":"Incomplete radiofrequency ablation promotes the development of CD133+ cancer stem cells in hepatocellular carcinoma cell line HepG2 via inducing SOX9 expression","author_short":["Yuan, C.","Wang, Z.","Liu, K.","Liu, D."],"year":2018,"bibtype":"article","biburl":"https://bibbase.org/zotero/sannpeterson","bibdata":{"bibtype":"article","type":"article","title":"Incomplete radiofrequency ablation promotes the development of CD133+ cancer stem cells in hepatocellular carcinoma cell line HepG2 via inducing SOX9 expression","issn":"1499-3872","url":"http://www.sciencedirect.com/science/article/pii/S1499387218302029","doi":"10.1016/j.hbpd.2018.09.012","abstract":"Background Cancer stem cells (CSCs) accelerate the growth of hepatocellular carcinoma (HCC) residual after incomplete radiofrequency ablation (In-RFA). The present study aimed to detect the effects of In-RFA on stemness transcription factors (STFs) expression which are important for the production and function of CSCs, and to find which STFs promote HCC stemness after In-RFA. Methods HepG2 cells were used for in vitro and in vivo studies. Flow cytometry and sphere-formation assays were used to detect the level and function of CD133+CSCs in the models. PCR array and ELISA were applied to analyze the altered expression of 84 STFs in CD133+CSCs in two models. Specific lentiviral shRNA was used to knockdown STFs expression, followed by detecting In-RFA's effects on the levels and function of CD133+CSCs. Results In-RFA was identified to induce CD133+CSCs and increase their tumorigenesis ability in vitro and in vivo. The mRNA levels of 84 STFs in CD133+CSCs were detected by PCR array, showing that 15 and 22 STFs were up-regulated in two models, respectively. Meanwhile, the mRNA levels of seven common STFs were up-regulated in both models. ELISA assay demonstrated that only the protein of sex determining region Y-box 9 (SOX9) was up-regulated in both models, the protein levels of the other 6 common STFs did not increase in both models. Finally, SOX9 was identified to play an important role in inducing, maintaining stemness and promoting tumorigenesis ability of CD133+CSCs in both models. Conclusion In-RFA-induced SOX9 stimulates CD133+CSCs proliferation and increases their tumorigenesis ability, suggesting that SOX9 may be a good target for HCC treatment.","urldate":"2018-09-29","journal":"Hepatobiliary & Pancreatic Diseases International","author":[{"propositions":[],"lastnames":["Yuan"],"firstnames":["Chun-Wang"],"suffixes":[]},{"propositions":[],"lastnames":["Wang"],"firstnames":["Zhen-Chang"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Kai"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Dong-Jie"],"suffixes":[]}],"month":"September","year":"2018","keywords":"Cancer stem cells, HepG2, Hepatocellular carcinoma, Radiofrequency ablation","bibtex":"@article{yuan_incomplete_2018,\n\ttitle = {Incomplete radiofrequency ablation promotes the development of {CD133}+ cancer stem cells in hepatocellular carcinoma cell line {HepG2} via inducing {SOX9} expression},\n\tissn = {1499-3872},\n\turl = {http://www.sciencedirect.com/science/article/pii/S1499387218302029},\n\tdoi = {10.1016/j.hbpd.2018.09.012},\n\tabstract = {Background\nCancer stem cells (CSCs) accelerate the growth of hepatocellular carcinoma (HCC) residual after incomplete radiofrequency ablation (In-RFA). The present study aimed to detect the effects of In-RFA on stemness transcription factors (STFs) expression which are important for the production and function of CSCs, and to find which STFs promote HCC stemness after In-RFA.\nMethods\nHepG2 cells were used for in vitro and in vivo studies. Flow cytometry and sphere-formation assays were used to detect the level and function of CD133+CSCs in the models. PCR array and ELISA were applied to analyze the altered expression of 84 STFs in CD133+CSCs in two models. Specific lentiviral shRNA was used to knockdown STFs expression, followed by detecting In-RFA's effects on the levels and function of CD133+CSCs.\nResults\nIn-RFA was identified to induce CD133+CSCs and increase their tumorigenesis ability in vitro and in vivo. The mRNA levels of 84 STFs in CD133+CSCs were detected by PCR array, showing that 15 and 22 STFs were up-regulated in two models, respectively. Meanwhile, the mRNA levels of seven common STFs were up-regulated in both models. ELISA assay demonstrated that only the protein of sex determining region Y-box 9 (SOX9) was up-regulated in both models, the protein levels of the other 6 common STFs did not increase in both models. Finally, SOX9 was identified to play an important role in inducing, maintaining stemness and promoting tumorigenesis ability of CD133+CSCs in both models.\nConclusion\nIn-RFA-induced SOX9 stimulates CD133+CSCs proliferation and increases their tumorigenesis ability, suggesting that SOX9 may be a good target for HCC treatment.},\n\turldate = {2018-09-29},\n\tjournal = {Hepatobiliary \\& Pancreatic Diseases International},\n\tauthor = {Yuan, Chun-Wang and Wang, Zhen-Chang and Liu, Kai and Liu, Dong-Jie},\n\tmonth = sep,\n\tyear = {2018},\n\tkeywords = {Cancer stem cells, HepG2, Hepatocellular carcinoma, Radiofrequency ablation},\n}\n\n","author_short":["Yuan, C.","Wang, Z.","Liu, K.","Liu, D."],"key":"yuan_incomplete_2018","id":"yuan_incomplete_2018","bibbaseid":"yuan-wang-liu-liu-incompleteradiofrequencyablationpromotesthedevelopmentofcd133cancerstemcellsinhepatocellularcarcinomacelllinehepg2viainducingsox9expression-2018","role":"author","urls":{"Paper":"http://www.sciencedirect.com/science/article/pii/S1499387218302029"},"keyword":["Cancer stem cells","HepG2","Hepatocellular carcinoma","Radiofrequency ablation"],"metadata":{"authorlinks":{}},"html":""},"search_terms":["incomplete","radiofrequency","ablation","promotes","development","cd133","cancer","stem","cells","hepatocellular","carcinoma","cell","line","hepg2","via","inducing","sox9","expression","yuan","wang","liu","liu"],"keywords":["cancer stem cells","hepg2","hepatocellular carcinoma","radiofrequency ablation"],"authorIDs":[],"dataSources":["JPAoq8b4ppzXj5XYZ"]}