Methods for Lipase Immobilization and Their Use for Biodiesel Production from Vegetable Oil. Yucel, Y., Demir, C., Dizge, N., & Keskinler, B. ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS, 36(11):1203-1211, 2014.
abstract   bibtex   
In the present work, two different lipases (triacylglycerol hydrolase, EC 3.1.1.3), Lipozyme TL-100L and Novozyme 388, were immobilized onto three different low-cost supports using both adsorption and covalent method: celite 545, silica gel, and styrene-divinylbenzene copolymer. The maximum immobilization yield was obtained as 79.0% for Lipozyme TL-100L and the highest specific activity was 6.5 U/mg protein for Novozym 388. The properties of the support and immobilized derivatives were characterized by Fourier transform infrared spectroscopy. Maximum methyl esters yield was obtained as 98.3%. The lipases, which are immobilized by covalently, proved to be stable after even 10 repeated reuses.
@article{
 title = {Methods for Lipase Immobilization and Their Use for Biodiesel Production from Vegetable Oil},
 type = {article},
 year = {2014},
 identifiers = {[object Object]},
 pages = {1203-1211},
 volume = {36},
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 created = {2016-04-28T08:46:16.000Z},
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 last_modified = {2016-04-28T08:46:16.000Z},
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 abstract = {In the present work, two different lipases (triacylglycerol hydrolase,
EC 3.1.1.3), Lipozyme TL-100L and Novozyme 388, were immobilized onto
three different low-cost supports using both adsorption and covalent
method: celite 545, silica gel, and styrene-divinylbenzene copolymer.
The maximum immobilization yield was obtained as 79.0% for Lipozyme
TL-100L and the highest specific activity was 6.5 U/mg protein for
Novozym 388. The properties of the support and immobilized derivatives
were characterized by Fourier transform infrared spectroscopy. Maximum
methyl esters yield was obtained as 98.3%. The lipases, which are
immobilized by covalently, proved to be stable after even 10 repeated
reuses.},
 bibtype = {article},
 author = {Yucel, Y and Demir, C and Dizge, N and Keskinler, B},
 journal = {ENERGY SOURCES PART A-RECOVERY UTILIZATION AND ENVIRONMENTAL EFFECTS},
 number = {11}
}

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