Sequence distribution in microbial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-polyesters determined by NMR and MS. Zagar, E.; Krzan, A.; Adamus, G.; and Kowalczuk, M. Biomacromolecules, 7(7):2210–2216, 2006. doi abstract bibtex The microstructure of bacterial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters (PHBV) as well as a mixture of two PHBV copolyesters of different comonomer composition and sequence distribution was studied by 13C NMR based on dyad and triad analysis and multistage electrospray ionization mass spectrometry (ESI-MSn). Both techniques gave results that were in good agreement for all investigated samples. The effect of microstructure on PHBV thermal properties was investigated from the melting behavior of samples. A PHBV copolyester with randomly distributed hydroxyvalerate units (12.0 mol % HV) showed a single melting peak, whereas samples with nonrandom composition distribution showed multiple melting peaks in their thermograms. Such complex melting behavior suggested that the 12.9 and 27.1 mol % PHBV copolyesters were actually blends of several copolymers with widely different comonomer-unit composition.
@Article{zagar06sequence,
author = {Zagar, Ema and Krzan, Andrej and Adamus, Grazyna and Kowalczuk, Marek},
title = {Sequence distribution in microbial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) co-polyesters determined by NMR and MS.},
journal = {Biomacromolecules},
year = {2006},
volume = {7},
number = {7},
pages = {2210--2216},
abstract = {The microstructure of bacterial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolyesters (PHBV) as well as a mixture of two PHBV copolyesters of different comonomer composition and sequence distribution was studied by 13C NMR based on dyad and triad analysis and multistage electrospray ionization mass spectrometry (ESI-MSn). Both techniques gave results that were in good agreement for all investigated samples. The effect of microstructure on PHBV thermal properties was investigated from the melting behavior of samples. A PHBV copolyester with randomly distributed hydroxyvalerate units (12.0 mol \% HV) showed a single melting peak, whereas samples with nonrandom composition distribution showed multiple melting peaks in their thermograms. Such complex melting behavior suggested that the 12.9 and 27.1 mol \% PHBV copolyesters were actually blends of several copolymers with widely different comonomer-unit composition.},
doi = {10.1021/bm060201g},
keywords = {Calorimetry, Differential Scanning; Cupriavidus necator, chemistry; Magnetic Resonance Spectroscopy; Molecular Structure; Polyesters, chemistry; Spectrometry, Mass, Electrospray Ionization; Temperature, polymer ms},
optmonth = jul,
owner = {martin},
pmid = {16827589},
timestamp = {2015.03.16},
}