Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots. Zhang, J., Eswaran, G., Alonso-Serra, J., Kucukoglu, M., Xiang, J., Yang, W., Elo, A., Nieminen, K., Damén, T., Joung, J., Yun, J., Lee, J., Ragni, L., Barbier de Reuille, P., Ahnert, S. E., Lee, J., Mähönen, A. P., & Helariutta, Y. Nature Plants, 5(10):1033–1042, October, 2019. Publisher: Nature Publishing Group![Transcriptional regulatory framework for vascular cambium development in Arabidopsis roots [link]](https://bibbase.org/img/filetypes/link.svg "link")
Paper doi abstract bibtex Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4 and removal of the putative inhibitor PTL to engineer Arabidopsis for enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4 and PTL in cambium development.
@article{zhang_transcriptional_2019,
title = {Transcriptional regulatory framework for vascular cambium development in {Arabidopsis} roots},
volume = {5},
copyright = {2019 The Author(s), under exclusive licence to Springer Nature Limited},
issn = {2055-0278},
url = {https://www.nature.com/articles/s41477-019-0522-9},
doi = {10.1038/s41477-019-0522-9},
abstract = {Vascular cambium, a lateral plant meristem, is a central producer of woody biomass. Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). Using the transcription factor network information, we combined overexpression of the cambial activator WOX4 and removal of the putative inhibitor PTL to engineer Arabidopsis for enhanced radial growth. This line also showed ectopic cambial activity, thus further highlighting the central roles of WOX4 and PTL in cambium development.},
language = {en},
number = {10},
urldate = {2025-10-08},
journal = {Nature Plants},
author = {Zhang, Jing and Eswaran, Gugan and Alonso-Serra, Juan and Kucukoglu, Melis and Xiang, Jiale and Yang, Weibing and Elo, Annakaisa and Nieminen, Kaisa and Damén, Teddy and Joung, Je-Gun and Yun, Jae-Young and Lee, Jung-Hun and Ragni, Laura and Barbier de Reuille, Pierre and Ahnert, Sebastian E. and Lee, Ji-Young and Mähönen, Ari Pekka and Helariutta, Ykä},
month = oct,
year = {2019},
note = {Publisher: Nature Publishing Group},
keywords = {Plant development, Plant genetics},
pages = {1033--1042},
}
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Although a few transcription factors have been shown to regulate cambial activity1, the phenotypes of the corresponding loss-of-function mutants are relatively modest, highlighting our limited understanding of the underlying transcriptional regulation. Here, we use cambium cell-specific transcript profiling followed by a combination of transcription factor network and genetic analyses to identify 62 new transcription factor genotypes displaying an array of cambial phenotypes. This approach culminated in virtual loss of cambial activity when both WUSCHEL-RELATED HOMEOBOX 4 (WOX4) and KNOTTED-like from Arabidopsis thaliana 1 (KNAT1; also known as BREVIPEDICELLUS) were mutated, thereby unlocking the genetic redundancy in the regulation of cambium development. We also identified transcription factors with dual functions in cambial cell proliferation and xylem differentiation, including WOX4, SHORT VEGETATIVE PHASE (SVP) and PETAL LOSS (PTL). 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