Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog. Zhang, Y., Liu, G., Guo, T., Liang, X. G, Du, H., Yang, L., Bhaduri, A., Li, X., Xu, Z., Zhang, Z., Li, Z., He, M., Tsyporin, J., Kriegstein, A. R, Rubenstein, J. L, Yang, Z., & Chen, B. Cell Rep, 30(13):4490–4504.e4, March, 2020. abstract bibtex Neural stem cells (NSCs) in the prenatal neocortex progressively generate different subtypes of glutamatergic projection neurons. Following that, NSCs have a major switch in their progenitor properties and produce $γ$-aminobutyric acid (GABAergic) interneurons for the olfactory bulb (OB), cortical oligodendrocytes, and astrocytes. Herein, we provide evidence for the molecular mechanism that underlies this switch in the state of neocortical NSCs. We show that, at around E16.5, mouse neocortical NSCs start to generate GSX2-expressing (GSX2(+)) intermediate progenitor cells (IPCs). In vivo lineage-tracing study revealed that GSX2(+) IPC population gives rise not only to OB interneurons but also to cortical oligodendrocytes and astrocytes, suggesting that they are a tri-potential population. We demonstrated that Sonic hedgehog signaling is both necessary and sufficient for the generation of GSX2(+) IPCs by reducing GLI3R protein levels. Using single-cell RNA sequencing, we identify the transcriptional profile of GSX2(+) IPCs and the process of the lineage switch of cortical NSCs.
@ARTICLE{Zhang2020-ie,
title = "Cortical Neural Stem Cell Lineage Progression Is Regulated by
Extrinsic Signaling Molecule Sonic Hedgehog",
author = "Zhang, Yue and Liu, Guoping and Guo, Teng and Liang, Xiaoyi G and
Du, Heng and Yang, Lin and Bhaduri, Aparna and Li, Xiaosu and Xu,
Zhejun and Zhang, Zhuangzhi and Li, Zhenmeiyu and He, Miao and
Tsyporin, Jeremiah and Kriegstein, Arnold R and Rubenstein, John
L and Yang, Zhengang and Chen, Bin",
abstract = "Neural stem cells (NSCs) in the prenatal neocortex progressively
generate different subtypes of glutamatergic projection neurons.
Following that, NSCs have a major switch in their progenitor
properties and produce $\gamma$-aminobutyric acid (GABAergic)
interneurons for the olfactory bulb (OB), cortical
oligodendrocytes, and astrocytes. Herein, we provide evidence for
the molecular mechanism that underlies this switch in the state
of neocortical NSCs. We show that, at around E16.5, mouse
neocortical NSCs start to generate GSX2-expressing (GSX2(+))
intermediate progenitor cells (IPCs). In vivo lineage-tracing
study revealed that GSX2(+) IPC population gives rise not only to
OB interneurons but also to cortical oligodendrocytes and
astrocytes, suggesting that they are a tri-potential population.
We demonstrated that Sonic hedgehog signaling is both necessary
and sufficient for the generation of GSX2(+) IPCs by reducing
GLI3R protein levels. Using single-cell RNA sequencing, we
identify the transcriptional profile of GSX2(+) IPCs and the
process of the lineage switch of cortical NSCs.",
journal = "Cell Rep",
volume = 30,
number = 13,
pages = "4490--4504.e4",
month = mar,
year = 2020,
keywords = "Gli3; Gsx2; Shh; cerebral cortex; neural stem cells; olfactory
bulb interneurons; oligodendrocytes",
language = "en"
}
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L","Yang, Z.","Chen, B."],"bibdata":{"bibtype":"article","type":"article","title":"Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog","author":[{"propositions":[],"lastnames":["Zhang"],"firstnames":["Yue"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Guoping"],"suffixes":[]},{"propositions":[],"lastnames":["Guo"],"firstnames":["Teng"],"suffixes":[]},{"propositions":[],"lastnames":["Liang"],"firstnames":["Xiaoyi","G"],"suffixes":[]},{"propositions":[],"lastnames":["Du"],"firstnames":["Heng"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Lin"],"suffixes":[]},{"propositions":[],"lastnames":["Bhaduri"],"firstnames":["Aparna"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Xiaosu"],"suffixes":[]},{"propositions":[],"lastnames":["Xu"],"firstnames":["Zhejun"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Zhuangzhi"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Zhenmeiyu"],"suffixes":[]},{"propositions":[],"lastnames":["He"],"firstnames":["Miao"],"suffixes":[]},{"propositions":[],"lastnames":["Tsyporin"],"firstnames":["Jeremiah"],"suffixes":[]},{"propositions":[],"lastnames":["Kriegstein"],"firstnames":["Arnold","R"],"suffixes":[]},{"propositions":[],"lastnames":["Rubenstein"],"firstnames":["John","L"],"suffixes":[]},{"propositions":[],"lastnames":["Yang"],"firstnames":["Zhengang"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Bin"],"suffixes":[]}],"abstract":"Neural stem cells (NSCs) in the prenatal neocortex progressively generate different subtypes of glutamatergic projection neurons. Following that, NSCs have a major switch in their progenitor properties and produce $γ$-aminobutyric acid (GABAergic) interneurons for the olfactory bulb (OB), cortical oligodendrocytes, and astrocytes. Herein, we provide evidence for the molecular mechanism that underlies this switch in the state of neocortical NSCs. We show that, at around E16.5, mouse neocortical NSCs start to generate GSX2-expressing (GSX2(+)) intermediate progenitor cells (IPCs). In vivo lineage-tracing study revealed that GSX2(+) IPC population gives rise not only to OB interneurons but also to cortical oligodendrocytes and astrocytes, suggesting that they are a tri-potential population. We demonstrated that Sonic hedgehog signaling is both necessary and sufficient for the generation of GSX2(+) IPCs by reducing GLI3R protein levels. Using single-cell RNA sequencing, we identify the transcriptional profile of GSX2(+) IPCs and the process of the lineage switch of cortical NSCs.","journal":"Cell Rep","volume":"30","number":"13","pages":"4490–4504.e4","month":"March","year":"2020","keywords":"Gli3; Gsx2; Shh; cerebral cortex; neural stem cells; olfactory bulb interneurons; oligodendrocytes","language":"en","bibtex":"@ARTICLE{Zhang2020-ie,\n title = \"Cortical Neural Stem Cell Lineage Progression Is Regulated by\n Extrinsic Signaling Molecule Sonic Hedgehog\",\n author = \"Zhang, Yue and Liu, Guoping and Guo, Teng and Liang, Xiaoyi G and\n Du, Heng and Yang, Lin and Bhaduri, Aparna and Li, Xiaosu and Xu,\n Zhejun and Zhang, Zhuangzhi and Li, Zhenmeiyu and He, Miao and\n Tsyporin, Jeremiah and Kriegstein, Arnold R and Rubenstein, John\n L and Yang, Zhengang and Chen, Bin\",\n abstract = \"Neural stem cells (NSCs) in the prenatal neocortex progressively\n generate different subtypes of glutamatergic projection neurons.\n Following that, NSCs have a major switch in their progenitor\n properties and produce $\\gamma$-aminobutyric acid (GABAergic)\n interneurons for the olfactory bulb (OB), cortical\n oligodendrocytes, and astrocytes. Herein, we provide evidence for\n the molecular mechanism that underlies this switch in the state\n of neocortical NSCs. We show that, at around E16.5, mouse\n neocortical NSCs start to generate GSX2-expressing (GSX2(+))\n intermediate progenitor cells (IPCs). In vivo lineage-tracing\n study revealed that GSX2(+) IPC population gives rise not only to\n OB interneurons but also to cortical oligodendrocytes and\n astrocytes, suggesting that they are a tri-potential population.\n We demonstrated that Sonic hedgehog signaling is both necessary\n and sufficient for the generation of GSX2(+) IPCs by reducing\n GLI3R protein levels. Using single-cell RNA sequencing, we\n identify the transcriptional profile of GSX2(+) IPCs and the\n process of the lineage switch of cortical NSCs.\",\n journal = \"Cell Rep\",\n volume = 30,\n number = 13,\n pages = \"4490--4504.e4\",\n month = mar,\n year = 2020,\n keywords = \"Gli3; Gsx2; Shh; cerebral cortex; neural stem cells; olfactory\n bulb interneurons; oligodendrocytes\",\n language = \"en\"\n}\n\n","author_short":["Zhang, Y.","Liu, G.","Guo, T.","Liang, X. G","Du, H.","Yang, L.","Bhaduri, A.","Li, X.","Xu, Z.","Zhang, Z.","Li, Z.","He, M.","Tsyporin, J.","Kriegstein, A. R","Rubenstein, J. L","Yang, Z.","Chen, B."],"key":"Zhang2020-ie","id":"Zhang2020-ie","bibbaseid":"zhang-liu-guo-liang-du-yang-bhaduri-li-etal-corticalneuralstemcelllineageprogressionisregulatedbyextrinsicsignalingmoleculesonichedgehog-2020","role":"author","urls":{},"keyword":["Gli3; Gsx2; Shh; cerebral cortex; neural stem cells; olfactory bulb interneurons; oligodendrocytes"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/f/EJMp3HRuxirjxpcXh/references.bib","dataSources":["sAFYeB74DpbdXM9NN","4zx9n2tbeLTix3Wxr","k3cdWrThyTh5o59Rm","hq9pebjzmsTuyxGGx","h8Atv2SAy4PmShg5j"],"keywords":["gli3; gsx2; shh; cerebral cortex; neural stem cells; olfactory bulb interneurons; oligodendrocytes"],"search_terms":["cortical","neural","stem","cell","lineage","progression","regulated","extrinsic","signaling","molecule","sonic","hedgehog","zhang","liu","guo","liang","du","yang","bhaduri","li","xu","zhang","li","he","tsyporin","kriegstein","rubenstein","yang","chen"],"title":"Cortical Neural Stem Cell Lineage Progression Is Regulated by Extrinsic Signaling Molecule Sonic Hedgehog","year":2020}