@article{zhang_inhibition_2012,
	title = {Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines {CaOV}3 and {SKOV}3 by natural withanolide {Withaferin} {A}},
	volume = {124},
	issn = {1095-6859},
	doi = {10.1016/j.ygyno.2011.11.044},
	abstract = {OBJECTIVE: Withaferin A, a natural withanolide, has shown anti-cancer properties in various cancers including breast cancer, but its effects in ovarian cancer remain unexplored. Notch 1 and Notch3 are critically involved in ovarian cancer progression. We decided to examine the effects of Withaferin A in ovarian carcinoma cell lines and its molecular mechanism of action including its regulation of Notch.
METHODS: The effects of Withaferin A were examined in CaOV3 and SKOV3 ovarian carcinoma cell lines using MTS assay, clonogenic assay, annexin V/propidium iodide flow cytometry, and cell cycle analysis. Western analysis was conducted to examine the molecular mechanisms of action.
RESULTS: Withaferin A inhibited the growth and colony formation of CaOV3 and SKOV3 cells by inducing apoptosis and cell cycle arrest. These changes correlated with down-regulation of Notch1, Notch3, cdc25C, total and phosphorylated Akt, and bcl-2 proteins.
CONCLUSIONS: Withaferin A inhibits CaOV3 and SKOV3 ovarian carcinoma cell growth, at least in part by targeting Notch1 and Notch3.},
	language = {eng},
	number = {3},
	journal = {Gynecologic Oncology},
	author = {Zhang, Xuan and Samadi, Abbas K. and Roby, Katherine F. and Timmermann, Barbara and Cohen, Mark S.},
	month = mar,
	year = {2012},
	pmid = {22188785},
	pmcid = {PMC3278582},
	keywords = {Antineoplastic Agents, Apoptosis, Cell Growth Processes, Down-Regulation, Female, G2 Phase Cell Cycle Checkpoints, Humans, M Phase Cell Cycle Checkpoints, Oncogene Protein v-akt, Ovarian Neoplasms, Proto-Oncogene Proteins c-bcl-2, Receptor, Notch1, Receptor, Notch3, Receptors, Notch, Withanolides, cdc25 Phosphatases},
	pages = {606--612}
}

{"_id":"Ci99QMcZwpv7ZD7S7","bibbaseid":"zhang-samadi-roby-timmermann-cohen-inhibitionofcellgrowthandinductionofapoptosisinovariancarcinomacelllinescaov3andskov3bynaturalwithanolidewithaferina-2012","downloads":0,"creationDate":"2018-04-17T15:45:25.361Z","title":"Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A","author_short":["Zhang, X.","Samadi, A. K.","Roby, K. F.","Timmermann, B.","Cohen, M. S."],"year":2012,"bibtype":"article","biburl":"https://api.zotero.org/groups/917610/items?key=5Tpdn4Dba9sBkYSJL5LXwhhi&format=bibtex&limit=100","bibdata":{"bibtype":"article","type":"article","title":"Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines CaOV3 and SKOV3 by natural withanolide Withaferin A","volume":"124","issn":"1095-6859","doi":"10.1016/j.ygyno.2011.11.044","abstract":"OBJECTIVE: Withaferin A, a natural withanolide, has shown anti-cancer properties in various cancers including breast cancer, but its effects in ovarian cancer remain unexplored. Notch 1 and Notch3 are critically involved in ovarian cancer progression. We decided to examine the effects of Withaferin A in ovarian carcinoma cell lines and its molecular mechanism of action including its regulation of Notch. METHODS: The effects of Withaferin A were examined in CaOV3 and SKOV3 ovarian carcinoma cell lines using MTS assay, clonogenic assay, annexin V/propidium iodide flow cytometry, and cell cycle analysis. Western analysis was conducted to examine the molecular mechanisms of action. RESULTS: Withaferin A inhibited the growth and colony formation of CaOV3 and SKOV3 cells by inducing apoptosis and cell cycle arrest. These changes correlated with down-regulation of Notch1, Notch3, cdc25C, total and phosphorylated Akt, and bcl-2 proteins. CONCLUSIONS: Withaferin A inhibits CaOV3 and SKOV3 ovarian carcinoma cell growth, at least in part by targeting Notch1 and Notch3.","language":"eng","number":"3","journal":"Gynecologic Oncology","author":[{"propositions":[],"lastnames":["Zhang"],"firstnames":["Xuan"],"suffixes":[]},{"propositions":[],"lastnames":["Samadi"],"firstnames":["Abbas","K."],"suffixes":[]},{"propositions":[],"lastnames":["Roby"],"firstnames":["Katherine","F."],"suffixes":[]},{"propositions":[],"lastnames":["Timmermann"],"firstnames":["Barbara"],"suffixes":[]},{"propositions":[],"lastnames":["Cohen"],"firstnames":["Mark","S."],"suffixes":[]}],"month":"March","year":"2012","pmid":"22188785","pmcid":"PMC3278582","keywords":"Antineoplastic Agents, Apoptosis, Cell Growth Processes, Down-Regulation, Female, G2 Phase Cell Cycle Checkpoints, Humans, M Phase Cell Cycle Checkpoints, Oncogene Protein v-akt, Ovarian Neoplasms, Proto-Oncogene Proteins c-bcl-2, Receptor, Notch1, Receptor, Notch3, Receptors, Notch, Withanolides, cdc25 Phosphatases","pages":"606--612","bibtex":"@article{zhang_inhibition_2012,\n\ttitle = {Inhibition of cell growth and induction of apoptosis in ovarian carcinoma cell lines {CaOV}3 and {SKOV}3 by natural withanolide {Withaferin} {A}},\n\tvolume = {124},\n\tissn = {1095-6859},\n\tdoi = {10.1016/j.ygyno.2011.11.044},\n\tabstract = {OBJECTIVE: Withaferin A, a natural withanolide, has shown anti-cancer properties in various cancers including breast cancer, but its effects in ovarian cancer remain unexplored. Notch 1 and Notch3 are critically involved in ovarian cancer progression. We decided to examine the effects of Withaferin A in ovarian carcinoma cell lines and its molecular mechanism of action including its regulation of Notch.\nMETHODS: The effects of Withaferin A were examined in CaOV3 and SKOV3 ovarian carcinoma cell lines using MTS assay, clonogenic assay, annexin V/propidium iodide flow cytometry, and cell cycle analysis. Western analysis was conducted to examine the molecular mechanisms of action.\nRESULTS: Withaferin A inhibited the growth and colony formation of CaOV3 and SKOV3 cells by inducing apoptosis and cell cycle arrest. These changes correlated with down-regulation of Notch1, Notch3, cdc25C, total and phosphorylated Akt, and bcl-2 proteins.\nCONCLUSIONS: Withaferin A inhibits CaOV3 and SKOV3 ovarian carcinoma cell growth, at least in part by targeting Notch1 and Notch3.},\n\tlanguage = {eng},\n\tnumber = {3},\n\tjournal = {Gynecologic Oncology},\n\tauthor = {Zhang, Xuan and Samadi, Abbas K. and Roby, Katherine F. and Timmermann, Barbara and Cohen, Mark S.},\n\tmonth = mar,\n\tyear = {2012},\n\tpmid = {22188785},\n\tpmcid = {PMC3278582},\n\tkeywords = {Antineoplastic Agents, Apoptosis, Cell Growth Processes, Down-Regulation, Female, G2 Phase Cell Cycle Checkpoints, Humans, M Phase Cell Cycle Checkpoints, Oncogene Protein v-akt, Ovarian Neoplasms, Proto-Oncogene Proteins c-bcl-2, Receptor, Notch1, Receptor, Notch3, Receptors, Notch, Withanolides, cdc25 Phosphatases},\n\tpages = {606--612}\n}\n\n","author_short":["Zhang, X.","Samadi, A. K.","Roby, K. F.","Timmermann, B.","Cohen, M. 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