Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803. Zhang, L., Selao, T. T., Selstam, E., & Norling, B. PLoS One, 10(6):e0130904, June, 2015. Edition: 2015/06/18Paper doi abstract bibtex The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of beta-carotene precursor molecules and echinenone.
@article{zhang_subcellular_2015,
title = {Subcellular {Localization} of {Carotenoid} {Biosynthesis} in {Synechocystis} sp. {PCC} 6803},
volume = {10},
issn = {1932-6203 (Electronic) 1932-6203 (Linking)},
url = {https://www.ncbi.nlm.nih.gov/pubmed/26083372},
doi = {10.1371/journal.pone.0130904},
abstract = {The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of beta-carotene precursor molecules and echinenone.},
language = {en},
number = {6},
urldate = {2021-06-07},
journal = {PLoS One},
author = {Zhang, L. and Selao, T. T. and Selstam, E. and Norling, B.},
month = jun,
year = {2015},
note = {Edition: 2015/06/18},
keywords = {Bacterial Proteins/*metabolism, Biosynthetic Pathways, Carotenoids/*biosynthesis, Cell Membrane/*chemistry/metabolism, Chromatography, High Pressure Liquid, Subcellular Fractions, Synechocystis/growth \& development/*metabolism},
pages = {e0130904},
}
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