Haplotype-resolved genome assembly of Coriaria nepalensis a non-legume nitrogen-fixing shrub. Zhao, S., Guo, J., Kong, L., Nie, S., Yan, X., Shi, T., Tian, X., Ma, H., Bao, Y., Li, Z., Chen, Z., Zhang, R., Ma, Y., El-Kassaby, Y. A., Porth, I., Zhao, W., & Mao, J. Scientific Data, 10(1):259, May, 2023. Number: 1 Publisher: Nature Publishing GroupPaper doi abstract bibtex Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.
@article{zhao_haplotype-resolved_2023,
title = {Haplotype-resolved genome assembly of {Coriaria} nepalensis a non-legume nitrogen-fixing shrub},
volume = {10},
copyright = {2023 The Author(s)},
issn = {2052-4463},
url = {https://www.nature.com/articles/s41597-023-02171-6},
doi = {10.1038/s41597-023-02171-6},
abstract = {Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9\% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5\% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.},
language = {en},
number = {1},
urldate = {2023-05-12},
journal = {Scientific Data},
author = {Zhao, Shi-Wei and Guo, Jing-Fang and Kong, Lei and Nie, Shuai and Yan, Xue-Mei and Shi, Tian-Le and Tian, Xue-Chan and Ma, Hai-Yao and Bao, Yu-Tao and Li, Zhi-Chao and Chen, Zhao-Yang and Zhang, Ren-Gang and Ma, Yong-Peng and El-Kassaby, Yousry A. and Porth, Ilga and Zhao, Wei and Mao, Jian-Feng},
month = may,
year = {2023},
note = {Number: 1
Publisher: Nature Publishing Group},
keywords = {Computational biology and bioinformatics, Plant sciences},
pages = {259},
}
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Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.","language":"en","number":"1","urldate":"2023-05-12","journal":"Scientific Data","author":[{"propositions":[],"lastnames":["Zhao"],"firstnames":["Shi-Wei"],"suffixes":[]},{"propositions":[],"lastnames":["Guo"],"firstnames":["Jing-Fang"],"suffixes":[]},{"propositions":[],"lastnames":["Kong"],"firstnames":["Lei"],"suffixes":[]},{"propositions":[],"lastnames":["Nie"],"firstnames":["Shuai"],"suffixes":[]},{"propositions":[],"lastnames":["Yan"],"firstnames":["Xue-Mei"],"suffixes":[]},{"propositions":[],"lastnames":["Shi"],"firstnames":["Tian-Le"],"suffixes":[]},{"propositions":[],"lastnames":["Tian"],"firstnames":["Xue-Chan"],"suffixes":[]},{"propositions":[],"lastnames":["Ma"],"firstnames":["Hai-Yao"],"suffixes":[]},{"propositions":[],"lastnames":["Bao"],"firstnames":["Yu-Tao"],"suffixes":[]},{"propositions":[],"lastnames":["Li"],"firstnames":["Zhi-Chao"],"suffixes":[]},{"propositions":[],"lastnames":["Chen"],"firstnames":["Zhao-Yang"],"suffixes":[]},{"propositions":[],"lastnames":["Zhang"],"firstnames":["Ren-Gang"],"suffixes":[]},{"propositions":[],"lastnames":["Ma"],"firstnames":["Yong-Peng"],"suffixes":[]},{"propositions":[],"lastnames":["El-Kassaby"],"firstnames":["Yousry","A."],"suffixes":[]},{"propositions":[],"lastnames":["Porth"],"firstnames":["Ilga"],"suffixes":[]},{"propositions":[],"lastnames":["Zhao"],"firstnames":["Wei"],"suffixes":[]},{"propositions":[],"lastnames":["Mao"],"firstnames":["Jian-Feng"],"suffixes":[]}],"month":"May","year":"2023","note":"Number: 1 Publisher: Nature Publishing Group","keywords":"Computational biology and bioinformatics, Plant sciences","pages":"259","bibtex":"@article{zhao_haplotype-resolved_2023,\n\ttitle = {Haplotype-resolved genome assembly of {Coriaria} nepalensis a non-legume nitrogen-fixing shrub},\n\tvolume = {10},\n\tcopyright = {2023 The Author(s)},\n\tissn = {2052-4463},\n\turl = {https://www.nature.com/articles/s41597-023-02171-6},\n\tdoi = {10.1038/s41597-023-02171-6},\n\tabstract = {Coriaria nepalensis Wall. (Coriariaceae) is a nitrogen-fixing shrub which forms root nodules with the actinomycete Frankia. Oils and extracts of C. nepalensis have been reported to be bacteriostatic and insecticidal, and C. nepalensis bark provides a valuable tannin resource. Here, by combining PacBio HiFi sequencing and Hi-C scaffolding techniques, we generated a haplotype-resolved chromosome-scale genome assembly for C. nepalensis. This genome assembly is approximately 620 Mb in size with a contig N50 of 11 Mb, with 99.9\\% of the total assembled sequences anchored to 40 pseudochromosomes. We predicted 60,862 protein-coding genes of which 99.5\\% were annotated from databases. We further identified 939 tRNAs, 7,297 rRNAs, and 982 ncRNAs. The chromosome-scale genome of C. nepalensis is expected to be a significant resource for understanding the genetic basis of root nodulation with Frankia, toxicity, and tannin biosynthesis.},\n\tlanguage = {en},\n\tnumber = {1},\n\turldate = {2023-05-12},\n\tjournal = {Scientific Data},\n\tauthor = {Zhao, Shi-Wei and Guo, Jing-Fang and Kong, Lei and Nie, Shuai and Yan, Xue-Mei and Shi, Tian-Le and Tian, Xue-Chan and Ma, Hai-Yao and Bao, Yu-Tao and Li, Zhi-Chao and Chen, Zhao-Yang and Zhang, Ren-Gang and Ma, Yong-Peng and El-Kassaby, Yousry A. and Porth, Ilga and Zhao, Wei and Mao, Jian-Feng},\n\tmonth = may,\n\tyear = {2023},\n\tnote = {Number: 1\nPublisher: Nature Publishing Group},\n\tkeywords = {Computational biology and bioinformatics, Plant sciences},\n\tpages = {259},\n}\n\n\n\n","author_short":["Zhao, S.","Guo, J.","Kong, L.","Nie, S.","Yan, X.","Shi, T.","Tian, X.","Ma, H.","Bao, Y.","Li, Z.","Chen, Z.","Zhang, R.","Ma, Y.","El-Kassaby, Y. 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