Multi-factorial modulation of colorectal carcinoma cells motility - partial coordination by the tetraspanin Co-029/tspan8. Zhu, Y., Ailane, N., Sala-Valdés, M., Haghighi-Rad, F., Billard, M., Nguyen, V., Saffroy, R., Lemoine, A., Rubinstein, E., Boucheix, C., & Greco, C. Oncotarget, 8(16):27454–27470, April, 2017. doi abstract bibtex Colorectal carcinoma cells Isreco1 display an ability to migrate controlled by a complex set of signals issued from the membrane. By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029). The modulation of cell migration linked to EGFR or CD44 can be neutralized by antagonizing Co-029 with the mAb Ts29.1 or by RNA interference. Altogether these data point to a crucial role of Co-029 in the modulation of colon cancer cell motility which could be related to the protumoral effect reported for this tetraspanin. Among surface molecules able to mediate Co-029 function, E-cadherin, EGFR and CD44 appear as likely candidates.
@article{zhu_multi-factorial_2017,
title = {Multi-factorial modulation of colorectal carcinoma cells motility - partial coordination by the tetraspanin {Co}-029/tspan8},
volume = {8},
issn = {1949-2553},
doi = {10.18632/oncotarget.16247},
abstract = {Colorectal carcinoma cells Isreco1 display an ability to migrate controlled by a complex set of signals issued from the membrane. By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029). The modulation of cell migration linked to EGFR or CD44 can be neutralized by antagonizing Co-029 with the mAb Ts29.1 or by RNA interference. Altogether these data point to a crucial role of Co-029 in the modulation of colon cancer cell motility which could be related to the protumoral effect reported for this tetraspanin. Among surface molecules able to mediate Co-029 function, E-cadherin, EGFR and CD44 appear as likely candidates.},
language = {eng},
number = {16},
journal = {Oncotarget},
author = {Zhu, Yingying and Ailane, Naouel and Sala-Valdés, Monica and Haghighi-Rad, Farhad and Billard, Martine and Nguyen, Viet and Saffroy, Raphael and Lemoine, Antoinette and Rubinstein, Eric and Boucheix, Claude and Greco, Céline},
month = apr,
year = {2017},
pmid = {28418857},
pmcid = {PMC5432348},
keywords = {Co-029/tspan8, EGFR, cell motility, colorectal carcinoma, mycoplasmas},
pages = {27454--27470},
}
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{"_id":"KwPpk9sJjoLRzHaXH","bibbaseid":"zhu-ailane-salavalds-haghighirad-billard-nguyen-saffroy-lemoine-etal-multifactorialmodulationofcolorectalcarcinomacellsmotilitypartialcoordinationbythetetraspaninco029tspan8-2017","author_short":["Zhu, Y.","Ailane, N.","Sala-Valdés, M.","Haghighi-Rad, F.","Billard, M.","Nguyen, V.","Saffroy, R.","Lemoine, A.","Rubinstein, E.","Boucheix, C.","Greco, C."],"bibdata":{"bibtype":"article","type":"article","title":"Multi-factorial modulation of colorectal carcinoma cells motility - partial coordination by the tetraspanin Co-029/tspan8","volume":"8","issn":"1949-2553","doi":"10.18632/oncotarget.16247","abstract":"Colorectal carcinoma cells Isreco1 display an ability to migrate controlled by a complex set of signals issued from the membrane. By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029). The modulation of cell migration linked to EGFR or CD44 can be neutralized by antagonizing Co-029 with the mAb Ts29.1 or by RNA interference. Altogether these data point to a crucial role of Co-029 in the modulation of colon cancer cell motility which could be related to the protumoral effect reported for this tetraspanin. 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By comparing cells infected by mycoplasmas and mycoplasmas free cells, we have established that basal 2D migration is dependent on a double signal mediated by the collagen receptors integrins alpha1/2 and the Toll-Like receptor TLR2. The signal issued from mycoplasmas can be replaced by a TLR2 ligand and the functional effect is neutralized by silencing of MyD88. Following previous observation that downregulation of E-cadherin/p120 catenin increases cell motility, we now report that EGFR or CD44 inhibition have a similar effect on cell motility that is restricted to tetraspanin Co-029/tspan8 transduced IsrecoI cells (Is1-Co029). The modulation of cell migration linked to EGFR or CD44 can be neutralized by antagonizing Co-029 with the mAb Ts29.1 or by RNA interference. Altogether these data point to a crucial role of Co-029 in the modulation of colon cancer cell motility which could be related to the protumoral effect reported for this tetraspanin. 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