@article{Zi-chang2024,
abstract = {BackgroundGinseng Radix et Rhizoma (GS) is frequently used as an adjuvant therapy for patients with heart failure (HF). Metoprolol is widely used in patients with HF. However, there is no report on the combined effects of GS and metoprolol in patients with HF.ObjectiveThis study investigated the combined effects of GS and metoprolol in male C57BL/6J mice with HF and the underlying mechanisms.Materials and methodsWe utilized a mouse myocardial HF model to measure the serum levels of creatine kinase (CK) and creatine kinase-MB form (CK-MB) using an automated biochemical analyzer. Lactate dehydrogenase (LDH) and cardiac troponin (cTnT) levels were determined using enzyme-linked immunosorbent assays. Autophagy of myocardial cells was evaluated using transmission electron microscopy, and changes in signal pathway proteins related to autophagy were analyzed by Western blotting.ResultsGS combined with metoprolol improved heart function, reduced heart damage, and decreased serum levels of CK, CK-MB, LDH, and cTnT. The combination of GS and metoprolol decreased autophagy in myocardial cells by reducing the levels of autophagy-related proteins (LC3, p62, Beclin1, and Atg5) and increasing the ratios of p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR.ConclusionGS enhanced the anti-heart failure effect of metoprolol. Its mechanism of action might be related to the inhibition of autophagy mediated by the activation of the PI3K/Akt/mTOR pathway.},
address = {San Francisco},
annote = {Name - Tianjin University of Traditional Chinese Medicine

Copyright - {\textcopyright} 2024 Zi-chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Last updated - 2024-10-03

SubjectsTermNotLitGenreText - Beijing China; United States--US; China; Social},
author = {Zi-chang, Niu and Xiao-ling, Han and Jin, Qi and Liu, Ting and Ming-hui, Ouyang and Hao-ping, Mao},
doi = {https://doi.org/10.1371/journal.pone.0301875},
journal = {PLoS One},
keywords = {1-Phosphatidylinositol 3-kinase,AKT protein,Autophagic cell death,Autophagy,Beijing China,Beta blockers,Beta-adrenergic antagonist therapy,Cardiomyopathy,Cardiovascular disease,Cardiovascular physiology,Cardiovascular therapy,China,Chinese medicine,Chromatography,Congestive heart failure,Creatine,Creatine kinase,Electron microscopy,Enzymes,Ginseng,Heart,Heart failure,Heart function,Immunoassays,Kinases,L-Lactate dehydrogenase,Laboratory animals,Lactate dehydrogenase,Males,Medical research,Metoprolol,Mitochondria,Mortality,Pharmaceuticals,Proteins,Receptor antagonist therapy,Sciences: Comprehensive Works,Serum levels,Social,TOR protein,Transmission electron microscopy,Troponin,United States--US,Western blotting},
language = {English},
month = {aug},
number = {8},
publisher = {Public Library of Science PP  - San Francisco},
title = {{Ginseng Radix et Rhizoma enhanced the effect of metoprolol in chronic heart failure by inhibiting autophagy in male C57BL/6J mice}},
url = {http://210.48.222.80/proxy.pac/scholarly-journals/i-ginseng-radix-et-rhizoma-enhanced-effect/docview/3093122540/se-2?accountid=44024 https://media.proquest.com/media/hms/PFT/1/HumVZ?_a=ChgyMDI0MTAxNTE1MDA1ODMzNDo0NTk4MzMSBTQ4NDczGgpPTkVfU0VBUkNIIg0yMTAuND},
volume = {19},
year = {2024}
}

{"_id":"aY94zpD2LLNm72ssM","bibbaseid":"zichang-xiaoling-jin-liu-minghui-haoping-ginsengradixetrhizomaenhancedtheeffectofmetoprololinchronicheartfailurebyinhibitingautophagyinmalec57bl6jmice-2024","author_short":["Zi-chang, N.","Xiao-ling, H.","Jin, Q.","Liu, T.","Ming-hui, O.","Hao-ping, M."],"bibdata":{"bibtype":"article","type":"article","abstract":"BackgroundGinseng Radix et Rhizoma (GS) is frequently used as an adjuvant therapy for patients with heart failure (HF). Metoprolol is widely used in patients with HF. However, there is no report on the combined effects of GS and metoprolol in patients with HF.ObjectiveThis study investigated the combined effects of GS and metoprolol in male C57BL/6J mice with HF and the underlying mechanisms.Materials and methodsWe utilized a mouse myocardial HF model to measure the serum levels of creatine kinase (CK) and creatine kinase-MB form (CK-MB) using an automated biochemical analyzer. Lactate dehydrogenase (LDH) and cardiac troponin (cTnT) levels were determined using enzyme-linked immunosorbent assays. Autophagy of myocardial cells was evaluated using transmission electron microscopy, and changes in signal pathway proteins related to autophagy were analyzed by Western blotting.ResultsGS combined with metoprolol improved heart function, reduced heart damage, and decreased serum levels of CK, CK-MB, LDH, and cTnT. The combination of GS and metoprolol decreased autophagy in myocardial cells by reducing the levels of autophagy-related proteins (LC3, p62, Beclin1, and Atg5) and increasing the ratios of p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR.ConclusionGS enhanced the anti-heart failure effect of metoprolol. Its mechanism of action might be related to the inhibition of autophagy mediated by the activation of the PI3K/Akt/mTOR pathway.","address":"San Francisco","annote":"Name - Tianjin University of Traditional Chinese Medicine Copyright - © 2024 Zi-chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Last updated - 2024-10-03 SubjectsTermNotLitGenreText - Beijing China; United States–US; China; Social","author":[{"propositions":[],"lastnames":["Zi-chang"],"firstnames":["Niu"],"suffixes":[]},{"propositions":[],"lastnames":["Xiao-ling"],"firstnames":["Han"],"suffixes":[]},{"propositions":[],"lastnames":["Jin"],"firstnames":["Qi"],"suffixes":[]},{"propositions":[],"lastnames":["Liu"],"firstnames":["Ting"],"suffixes":[]},{"propositions":[],"lastnames":["Ming-hui"],"firstnames":["Ouyang"],"suffixes":[]},{"propositions":[],"lastnames":["Hao-ping"],"firstnames":["Mao"],"suffixes":[]}],"doi":"https://doi.org/10.1371/journal.pone.0301875","journal":"PLoS One","keywords":"1-Phosphatidylinositol 3-kinase,AKT protein,Autophagic cell death,Autophagy,Beijing China,Beta blockers,Beta-adrenergic antagonist therapy,Cardiomyopathy,Cardiovascular disease,Cardiovascular physiology,Cardiovascular therapy,China,Chinese medicine,Chromatography,Congestive heart failure,Creatine,Creatine kinase,Electron microscopy,Enzymes,Ginseng,Heart,Heart failure,Heart function,Immunoassays,Kinases,L-Lactate dehydrogenase,Laboratory animals,Lactate dehydrogenase,Males,Medical research,Metoprolol,Mitochondria,Mortality,Pharmaceuticals,Proteins,Receptor antagonist therapy,Sciences: Comprehensive Works,Serum levels,Social,TOR protein,Transmission electron microscopy,Troponin,United States–US,Western blotting","language":"English","month":"aug","number":"8","publisher":"Public Library of Science PP - San Francisco","title":"Ginseng Radix et Rhizoma enhanced the effect of metoprolol in chronic heart failure by inhibiting autophagy in male C57BL/6J mice","url":"http://210.48.222.80/proxy.pac/scholarly-journals/i-ginseng-radix-et-rhizoma-enhanced-effect/docview/3093122540/se-2?accountid=44024 https://media.proquest.com/media/hms/PFT/1/HumVZ?_a=ChgyMDI0MTAxNTE1MDA1ODMzNDo0NTk4MzMSBTQ4NDczGgpPTkVfU0VBUkNIIg0yMTAuND","volume":"19","year":"2024","bibtex":"@article{Zi-chang2024,\nabstract = {BackgroundGinseng Radix et Rhizoma (GS) is frequently used as an adjuvant therapy for patients with heart failure (HF). Metoprolol is widely used in patients with HF. However, there is no report on the combined effects of GS and metoprolol in patients with HF.ObjectiveThis study investigated the combined effects of GS and metoprolol in male C57BL/6J mice with HF and the underlying mechanisms.Materials and methodsWe utilized a mouse myocardial HF model to measure the serum levels of creatine kinase (CK) and creatine kinase-MB form (CK-MB) using an automated biochemical analyzer. Lactate dehydrogenase (LDH) and cardiac troponin (cTnT) levels were determined using enzyme-linked immunosorbent assays. Autophagy of myocardial cells was evaluated using transmission electron microscopy, and changes in signal pathway proteins related to autophagy were analyzed by Western blotting.ResultsGS combined with metoprolol improved heart function, reduced heart damage, and decreased serum levels of CK, CK-MB, LDH, and cTnT. The combination of GS and metoprolol decreased autophagy in myocardial cells by reducing the levels of autophagy-related proteins (LC3, p62, Beclin1, and Atg5) and increasing the ratios of p-PI3K/PI3K, p-Akt/Akt, and p-mTOR/mTOR.ConclusionGS enhanced the anti-heart failure effect of metoprolol. Its mechanism of action might be related to the inhibition of autophagy mediated by the activation of the PI3K/Akt/mTOR pathway.},\naddress = {San Francisco},\nannote = {Name - Tianjin University of Traditional Chinese Medicine\n\nCopyright - {\\textcopyright} 2024 Zi-chang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License: http://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.\n\nLast updated - 2024-10-03\n\nSubjectsTermNotLitGenreText - Beijing China; United States--US; China; Social},\nauthor = {Zi-chang, Niu and Xiao-ling, Han and Jin, Qi and Liu, Ting and Ming-hui, Ouyang and Hao-ping, Mao},\ndoi = {https://doi.org/10.1371/journal.pone.0301875},\njournal = {PLoS One},\nkeywords = {1-Phosphatidylinositol 3-kinase,AKT protein,Autophagic cell death,Autophagy,Beijing China,Beta blockers,Beta-adrenergic antagonist therapy,Cardiomyopathy,Cardiovascular disease,Cardiovascular physiology,Cardiovascular therapy,China,Chinese medicine,Chromatography,Congestive heart failure,Creatine,Creatine kinase,Electron microscopy,Enzymes,Ginseng,Heart,Heart failure,Heart function,Immunoassays,Kinases,L-Lactate dehydrogenase,Laboratory animals,Lactate dehydrogenase,Males,Medical research,Metoprolol,Mitochondria,Mortality,Pharmaceuticals,Proteins,Receptor antagonist therapy,Sciences: Comprehensive Works,Serum levels,Social,TOR protein,Transmission electron microscopy,Troponin,United States--US,Western blotting},\nlanguage = {English},\nmonth = {aug},\nnumber = {8},\npublisher = {Public Library of Science PP - San Francisco},\ntitle = {{Ginseng Radix et Rhizoma enhanced the effect of metoprolol in chronic heart failure by inhibiting autophagy in male C57BL/6J mice}},\nurl = {http://210.48.222.80/proxy.pac/scholarly-journals/i-ginseng-radix-et-rhizoma-enhanced-effect/docview/3093122540/se-2?accountid=44024 https://media.proquest.com/media/hms/PFT/1/HumVZ?_a=ChgyMDI0MTAxNTE1MDA1ODMzNDo0NTk4MzMSBTQ4NDczGgpPTkVfU0VBUkNIIg0yMTAuND},\nvolume = {19},\nyear = {2024}\n}\n","author_short":["Zi-chang, N.","Xiao-ling, H.","Jin, Q.","Liu, T.","Ming-hui, O.","Hao-ping, M."],"key":"Zi-chang2024","id":"Zi-chang2024","bibbaseid":"zichang-xiaoling-jin-liu-minghui-haoping-ginsengradixetrhizomaenhancedtheeffectofmetoprololinchronicheartfailurebyinhibitingautophagyinmalec57bl6jmice-2024","role":"author","urls":{"Paper":"http://210.48.222.80/proxy.pac/scholarly-journals/i-ginseng-radix-et-rhizoma-enhanced-effect/docview/3093122540/se-2?accountid=44024 https://media.proquest.com/media/hms/PFT/1/HumVZ?_a=ChgyMDI0MTAxNTE1MDA1ODMzNDo0NTk4MzMSBTQ4NDczGgpPTkVfU0VBUkNIIg0yMTAuND"},"keyword":["1-Phosphatidylinositol 3-kinase","AKT protein","Autophagic cell death","Autophagy","Beijing China","Beta blockers","Beta-adrenergic antagonist therapy","Cardiomyopathy","Cardiovascular disease","Cardiovascular physiology","Cardiovascular therapy","China","Chinese medicine","Chromatography","Congestive heart failure","Creatine","Creatine kinase","Electron microscopy","Enzymes","Ginseng","Heart","Heart failure","Heart function","Immunoassays","Kinases","L-Lactate dehydrogenase","Laboratory animals","Lactate dehydrogenase","Males","Medical research","Metoprolol","Mitochondria","Mortality","Pharmaceuticals","Proteins","Receptor antagonist therapy","Sciences: Comprehensive Works","Serum levels","Social","TOR protein","Transmission electron microscopy","Troponin","United States–US","Western blotting"],"metadata":{"authorlinks":{}}},"bibtype":"article","biburl":"https://bibbase.org/network/files/yxMBZ4kDgB96wZJLd","dataSources":["iYKFbK3E7euWXFBxQ","ghn4Ru4eZwdLYsXAN"],"keywords":["1-phosphatidylinositol 3-kinase","akt protein","autophagic cell death","autophagy","beijing china","beta blockers","beta-adrenergic antagonist therapy","cardiomyopathy","cardiovascular disease","cardiovascular physiology","cardiovascular therapy","china","chinese medicine","chromatography","congestive heart failure","creatine","creatine kinase","electron microscopy","enzymes","ginseng","heart","heart failure","heart function","immunoassays","kinases","l-lactate dehydrogenase","laboratory animals","lactate dehydrogenase","males","medical research","metoprolol","mitochondria","mortality","pharmaceuticals","proteins","receptor antagonist therapy","sciences: comprehensive works","serum levels","social","tor protein","transmission electron microscopy","troponin","united states–us","western blotting"],"search_terms":["ginseng","radix","rhizoma","enhanced","effect","metoprolol","chronic","heart","failure","inhibiting","autophagy","male","c57bl","mice","zi-chang","xiao-ling","jin","liu","ming-hui","hao-ping"],"title":"Ginseng Radix et Rhizoma enhanced the effect of metoprolol in chronic heart failure by inhibiting autophagy in male C57BL/6J mice","year":2024}