Statistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae. Zybailov, B.; Mosley, A. L.; Sardiu, M. E.; Coleman, M. K.; Florens, L.; and Washburn, M. P. Journal of Proteome Research, 5(9):2339–2347, September, 2006.
Statistical Analysis of Membrane Proteome Expression Changes in Saccharomyces cerevisiae [link]Paper  doi  abstract   bibtex   
We have devised an approach for analyzing shotgun proteomics datasets based on the normalized spectral abundance factor that can be used for quantitative proteomics analysis. Three biological replicates of samples enriched for plasma membranes were isolated from S. cerevisiae grown in 14N-rich media and 15N-minimal media and analyzed via quantitative multidimensional protein identification technology. The natural log transformation of NSAF values from S. cerevisiae cells grown in 14N YPD media and 15N-minimal media had a normal distribution. The t-test analysis demonstrated 221 of 1316 proteins were significantly overexpressed in one or the other growth conditions with a p value \textless0.05. Notably, amino acid transporters were among the 14 membrane proteins that were significantly upregulated in cells grown in minimal media, and we functionally validated these increases in protein expression with radioisotope uptake assays for selected proteins. Keywords: quantitative proteomics • integral membrane proteins • statistical analysis • mass spectrometry • multidimensional protein identification technology
@article{zybailov_statistical_2006,
	title = {Statistical {Analysis} of {Membrane} {Proteome} {Expression} {Changes} in {Saccharomyces} cerevisiae},
	volume = {5},
	issn = {1535-3893},
	url = {http://dx.doi.org/10.1021/pr060161n},
	doi = {10.1021/pr060161n},
	abstract = {We have devised an approach for analyzing shotgun proteomics datasets based on the normalized spectral abundance factor that can be used for quantitative proteomics analysis. Three biological replicates of samples enriched for plasma membranes were isolated from S. cerevisiae grown in 14N-rich media and 15N-minimal media and analyzed via quantitative multidimensional protein identification technology. The natural log transformation of NSAF values from S. cerevisiae cells grown in 14N YPD media and 15N-minimal media had a normal distribution. The t-test analysis demonstrated 221 of 1316 proteins were significantly overexpressed in one or the other growth conditions with a p value {\textless}0.05. Notably, amino acid transporters were among the 14 membrane proteins that were significantly upregulated in cells grown in minimal media, and we functionally validated these increases in protein expression with radioisotope uptake assays for selected proteins. Keywords: quantitative proteomics • integral membrane proteins • statistical analysis • mass spectrometry • multidimensional protein identification technology},
	number = {9},
	urldate = {2016-11-28TZ},
	journal = {Journal of Proteome Research},
	author = {Zybailov, Boris and Mosley, Amber L. and Sardiu, Mihaela E. and Coleman, Michael K. and Florens, Laurence and Washburn, Michael P.},
	month = sep,
	year = {2006},
	pages = {2339--2347}
}
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