Arabinosylation Modulates the Growth-Regulating Activity of the Peptide Hormone CLE40a from Soybean. Corcilius, L., Hastwell, A., Zhang, M., Williams, J., Mackay, J., Gresshoff, P., Ferguson, B., & Payne, R. Cell Chemical Biology, 24(11):1347-1355.e7, 2017. doi abstract bibtex Small post-translationally modified peptide hormones mediate crucial developmental and regulatory processes in plants. CLAVATA/ENDOSPERM-SURROUNDING REGION (CLE) genes are found throughout the plant kingdom and encode for 12–13 amino acid peptides that must often undergo post-translational proline hydroxylation and glycosylation with O-β1,2-triarabinose moieties before they become functional. Apart from a few recent examples, a detailed understanding of the structure and function of most CLE hormones is yet to be uncovered. This is mainly owing to difficulties in isolating mature homogeneously modified CLE peptides from natural plant sources. In this study, we describe the efficient synthesis of a synthetic Araf 3 Hyp glycosylamino acid building block that was used to access a hitherto uninvestigated CLE hormone from soybean called GmCLE40a. Through the development and implementation of a novel in vivo root growth assay, we show that the synthetic triarabinosylated glycopeptide suppresses primary root growth in this important crop species. Herein Corcilius et al. describe an improved chemical synthesis of the β-O-tri-β1,2-arabinofuranosylated hydroxyproline post-translational modification present in plant glycopeptides and demonstrate its utility through the synthesis and biological evaluation of a novel root growth regulatory glycopeptide hormone, GmCLE40a, from soybean. The glycopeptide hormone displayed enhanced root growth suppressive activity in vivo compared with its unglycosylated isoform.
@article{
title = {Arabinosylation Modulates the Growth-Regulating Activity of the Peptide Hormone CLE40a from Soybean},
type = {article},
year = {2017},
pages = {1347-1355.e7},
volume = {24},
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abstract = {Small post-translationally modified peptide hormones mediate crucial developmental and regulatory processes in plants. CLAVATA/ENDOSPERM-SURROUNDING REGION (CLE) genes are found throughout the plant kingdom and encode for 12–13 amino acid peptides that must often undergo post-translational proline hydroxylation and glycosylation with O-β1,2-triarabinose moieties before they become functional. Apart from a few recent examples, a detailed understanding of the structure and function of most CLE hormones is yet to be uncovered. This is mainly owing to difficulties in isolating mature homogeneously modified CLE peptides from natural plant sources. In this study, we describe the efficient synthesis of a synthetic Araf 3 Hyp glycosylamino acid building block that was used to access a hitherto uninvestigated CLE hormone from soybean called GmCLE40a. Through the development and implementation of a novel in vivo root growth assay, we show that the synthetic triarabinosylated glycopeptide suppresses primary root growth in this important crop species. Herein Corcilius et al. describe an improved chemical synthesis of the β-O-tri-β1,2-arabinofuranosylated hydroxyproline post-translational modification present in plant glycopeptides and demonstrate its utility through the synthesis and biological evaluation of a novel root growth regulatory glycopeptide hormone, GmCLE40a, from soybean. The glycopeptide hormone displayed enhanced root growth suppressive activity in vivo compared with its unglycosylated isoform.},
bibtype = {article},
author = {Corcilius, L. and Hastwell, A.H. and Zhang, M. and Williams, J. and Mackay, J.P. and Gresshoff, P.M. and Ferguson, B.J. and Payne, R.J.},
doi = {10.1016/j.chembiol.2017.08.014},
journal = {Cell Chemical Biology},
number = {11}
}
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Herein Corcilius et al. describe an improved chemical synthesis of the β-O-tri-β1,2-arabinofuranosylated hydroxyproline post-translational modification present in plant glycopeptides and demonstrate its utility through the synthesis and biological evaluation of a novel root growth regulatory glycopeptide hormone, GmCLE40a, from soybean. 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