Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1. Gamsjaeger, R., Swanton, M., Kobus, F., Lehtomaki, E., Lowry, J., Kwan, A., Matthews, J., & Mackay, J. Journal of Biological Chemistry, 283(8):5158-5167, 2008.
doi  abstract   bibtex   
Zinc binding domains, or zinc fingers (ZnFs), form one of the most numerous and most diverse superclasses of protein structural motifs in eukaryotes. Although our understanding of the functions of several classes of these domains is relatively well developed, we know much less about the molecular mechanisms of action of many others. Myelin transcription factor 1 (MyT1) type ZnFs are found in organisms as diverse as nematodes and mammals and are found in a range of sequence contexts. MyT1, one of the early transcription factors expressed in the developing central nervous system, contains seven MyT1 ZnFs that are very highly conserved both within the protein and between species. We have used a range of biophysical techniques, including NMR spectroscopy and data-driven macromolecular docking, to investigate the structural basis for the interaction between MyT1 ZnFs and DNA. Our data indicate that MyT1 ZnFs recognize the major groove of DNA in a way that appears to differ from other known zinc binding domains. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
@article{
 title = {Structural and biophysical analysis of the DNA binding properties of myelin transcription factor 1},
 type = {article},
 year = {2008},
 pages = {5158-5167},
 volume = {283},
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 abstract = {Zinc binding domains, or zinc fingers (ZnFs), form one of the most numerous and most diverse superclasses of protein structural motifs in eukaryotes. Although our understanding of the functions of several classes of these domains is relatively well developed, we know much less about the molecular mechanisms of action of many others. Myelin transcription factor 1 (MyT1) type ZnFs are found in organisms as diverse as nematodes and mammals and are found in a range of sequence contexts. MyT1, one of the early transcription factors expressed in the developing central nervous system, contains seven MyT1 ZnFs that are very highly conserved both within the protein and between species. We have used a range of biophysical techniques, including NMR spectroscopy and data-driven macromolecular docking, to investigate the structural basis for the interaction between MyT1 ZnFs and DNA. Our data indicate that MyT1 ZnFs recognize the major groove of DNA in a way that appears to differ from other known zinc binding domains. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.},
 bibtype = {article},
 author = {Gamsjaeger, R. and Swanton, M.K. and Kobus, F.J. and Lehtomaki, E. and Lowry, J.A. and Kwan, A.H. and Matthews, J.M. and Mackay, J.P.},
 doi = {10.1074/jbc.M703772200},
 journal = {Journal of Biological Chemistry},
 number = {8}
}

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