Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1. Joseph, S., Kwan, A., Mackay, J., Cubeddu, L., & Matthews, J. Biomolecular NMR Assignments, 8(1):141-144, Kluwer Academic Publishers, 2014. cited By 3
Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1 [link]Paper  doi  abstract   bibtex   
The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4-DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins. © 2013 Springer Science+Business Media Dordrecht.
@ARTICLE{Joseph2014141,
author={Joseph, S. and Kwan, A.H.Y. and Mackay, J.P. and Cubeddu, L. and Matthews, J.M.},
title={Backbone and side-chain assignments of a tethered complex between LMO4 and DEAF-1},
journal={Biomolecular NMR Assignments},
year={2014},
volume={8},
number={1},
pages={141-144},
doi={10.1007/s12104-013-9470-x},
note={cited By 3},
url={https://www.scopus.com/inward/record.uri?eid=2-s2.0-84897113524&doi=10.1007%2fs12104-013-9470-x&partnerID=40&md5=c7124ed02817fd3e7ec09f2d053a081d},
affiliation={School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia; School of Science and Health, University of Western Sydney, Sydney, NSW 2751, Australia},
abstract={The transcriptional regulator LMO4 and the transcription factor DEAF-1 are both essential for brain and skeletal development. They are also implicated in human breast cancers; overexpression of LMO4 is an indicator of poor prognosis, and overexpression of DEAF-1 promotes epithelial breast cell proliferation. We have generated a stable LMO4-DEAF-1 complex comprising the C-terminal LIM domain of LMO4 and an intrinsically disordered LMO4-interaction domain from DEAF-1 tethered by a glycine/serine linker. Here we report the 1H, 15N and 13C assignments of this construct. Analysis of the assignments indicates the presence of structure in the DEAF-1 part of the complex supporting the presence of a physical interaction between the two proteins. © 2013 Springer Science+Business Media Dordrecht.},
author_keywords={Breast cancer;  DEAF-1;  Embryonic development;  LMO4;  Transcriptional complex},
keywords={Deaf1 protein, mouse;  LIM protein;  Lmo4 protein, mouse;  signal transducing adaptor protein;  transcription factor, amino acid sequence;  animal;  article;  chemistry;  human;  molecular genetics;  mouse;  nuclear magnetic resonance;  protein secondary structure, Adaptor Proteins, Signal Transducing;  Amino Acid Sequence;  Animals;  Humans;  LIM Domain Proteins;  Mice;  Molecular Sequence Data;  Nuclear Magnetic Resonance, Biomolecular;  Protein Structure, Secondary;  Transcription Factors},
correspondence_address1={Matthews, J.M.; School of Molecular Bioscience, University of Sydney, Sydney, NSW 2006, Australia; email: jacqui.matthews@sydney.edu.au},
publisher={Kluwer Academic Publishers},
issn={18742718},
pubmed_id={23417771},
language={English},
abbrev_source_title={Biomol. NMR Assignments},
document_type={Article},
source={Scopus},
}

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