The uncharacterized bacterial protein YejG has the same architecture as domain III of elongation factor G. Mohanty, B., Hanson-Manful, P., Finn, T., Chambers, C., McKellar, J., Macindoe, I., Helder, S., Setiyaputra, S., Zhong, Y., Mackay, J., Mackay, J., & Patrick, W. Proteins: Structure, Function and Bioinformatics, 87(8):699-705, 2019. doi abstract bibtex InterPro family IPR020489 comprises ~1000 uncharacterized bacterial proteins. Previously we showed that overexpressing the Escherichia coli representative of this family, EcYejG, conferred low-level resistance to aminoglycoside antibiotics. In an attempt to shed light on the biochemical function of EcYejG, we have solved its structure using multinuclear solution NMR spectroscopy. The structure most closely resembles that of domain III from elongation factor G (EF-G). EF-G catalyzes ribosomal translocation and mutations in EF-G have also been associated with aminoglycoside resistance. While we were unable to demonstrate a direct interaction between EcYejG and the ribosome, the protein might play a role in translation.
@article{
title = {The uncharacterized bacterial protein YejG has the same architecture as domain III of elongation factor G},
type = {article},
year = {2019},
pages = {699-705},
volume = {87},
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abstract = {InterPro family IPR020489 comprises ~1000 uncharacterized bacterial proteins. Previously we showed that overexpressing the Escherichia coli representative of this family, EcYejG, conferred low-level resistance to aminoglycoside antibiotics. In an attempt to shed light on the biochemical function of EcYejG, we have solved its structure using multinuclear solution NMR spectroscopy. The structure most closely resembles that of domain III from elongation factor G (EF-G). EF-G catalyzes ribosomal translocation and mutations in EF-G have also been associated with aminoglycoside resistance. While we were unable to demonstrate a direct interaction between EcYejG and the ribosome, the protein might play a role in translation.},
bibtype = {article},
author = {Mohanty, B. and Hanson-Manful, P. and Finn, T.J. and Chambers, C.R. and McKellar, J.L.O. and Macindoe, I. and Helder, S. and Setiyaputra, S. and Zhong, Y. and Mackay, J.P. and Mackay, J.P. and Patrick, W.M.},
doi = {10.1002/prot.25687},
journal = {Proteins: Structure, Function and Bioinformatics},
number = {8}
}
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